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Mucin-chitosan complexes at the solid-liquid interface: Multilayer formation and stability in surfactant solutions
YKI – Ytkemiska institutet.
YKI – Ytkemiska institutet.
2005 (engelsk)Inngår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 21, s. 9502-9509Artikkel i tidsskrift (Fagfellevurdert)
Abstract [en]

The adsorption of a biologically important glycoprotein, mucin, and mucin-chitosan complex layer formation on negatively charged surfaces, silica and mica, have been investigated employing ellipsometry, the interferometric surface apparatus, and atomic force microscopy techniques. Particular attention has been paid to the effect of an anionic surfactant sodium, dodecyl sulfate (SDS), with respect to the stability of the adsorption layers. It has been shown that mucin adsorbs on negatively charged surfaces to form highly hydrated layers. Such mucin layers readily associate with surfactants and are easily removed from the surfaces by rinsing with solutions of SDS at concentrations ≥0.2 cmc (1 cmc SDS in 30 mM NaCl is equal to 3.3 mM). The mucin adsorption layer is negatively charged, and we show how a positively charged polyelectrolyte, chitosan, associates with the preadsorbed mucin to form mucin-chitosan complexes that resist desorption by SDS even at SDS concentrations as high as 1 cmc. Thus, a method of mucin layer protection against removal by surfactants is offered. Further, we show how mucin-chitosan multilayers can be formed.

sted, utgiver, år, opplag, sider
2005. Vol. 21, s. 9502-9509
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Identifikatorer
URN: urn:nbn:se:ri:diva-27167OAI: oai:DiVA.org:ri-27167DiVA, id: diva2:1054171
Merknad
A1772Tilgjengelig fra: 2016-12-08 Laget: 2016-12-08 Sist oppdatert: 2017-11-29bibliografisk kontrollert

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