Studying solutions at high shear rates: A dedicated microfluidics setup Show others and affiliations
2016 (English) In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 23, no 2, p. 480-486Article in journal (Refereed) Published
Resource type Text
Abstract [en]
The development of a dedicated small-angle X-ray scattering setup for the investigation of complex fluids at different controlled shear conditions is reported. The setup utilizes a microfluidics chip with a narrowing channel. As a consequence, a shear gradient is generated within the channel and the effect of shear rate on structure and interactions is mapped spatially. In a first experiment small-angle X-ray scattering is utilized to investigate highly concentrated protein solutions up to a shear rate of 300000 s-1. These data demonstrate that equilibrium clusters of lysozyme are destabilized at high shear rates.
Place, publisher, year, edition, pages International Union of Crystallography , 2016. Vol. 23, no 2, p. 480-486
Keywords [en]
aggregation, beamline setup, complex solutions, lysozyme, microfluidics, protein interaction, rheology, shear, small-angle X-ray scattering (SAXS)
National Category
Physical Chemistry Other Chemistry Topics
Identifiers URN: urn:nbn:se:ri:diva-128 DOI: 10.1107/S1600577515024856 Scopus ID: 2-s2.0-84959274343 OAI: oai:DiVA.org:ri-128 DiVA, id: diva2:933951
2016-06-072016-05-312020-12-01 Bibliographically approved