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Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study
RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology. University of Gothenburg, Sweden.ORCID iD: 0000-0002-4270-8475
University of Gothenburg, Sweden.
University of Gothenburg, Sweden.
RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.ORCID iD: 0000-0002-8849-1793
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2024 (English)In: Journal of Orthopaedics, ISSN 0972-978X, E-ISSN 2589-9082, Vol. 47, p. 23-27Article in journal (Refereed) Published
Abstract [en]

Purpose: It is known that contact of nucleus pulposus with the dorsal root ganglion may induce changes in nerve conduction and pain behavior. It has also been suggested that the behavioristic changes are caused by changes in voltage-gated ion channels, which in turn have been upregulated by TNF. Such upregulations have previously been shown for NaV 1.8 and NaV 1.9. In this investigation, we expanded the number of studied ion channels after the application of nucleus pulposus or TNF. Methods: Following removal of the left L4-5 fact joint, a disc puncture was performed and the dorsal root ganglion was exposed to nucleus pulposus (n = 5) and TNF (n = 5). Operated rats without disc puncture served as sham (n = 5) and 5 non-operated (naïve) rats were included. After 24 h, the DRGs were harvested and analyzed by quantitative PCR on validated pre-spotted primer plates displaying genes for 90 voltage-gated ion channels. Results: It was evident that the changes in operated animals were separate from the naïve rats. It was also apparent that gene expression changes in rats with nucleus pulposus or TNF application showed similar trends and were also separated from sham-operated animals. Conclusion: The application of nucleus pulposus and TNF onto the DRG in rats induces comparable changes in gene expression of several ion channels. Since the changes induced by TNF and NP are similar, one might also suspect that TNF mediates the NP-induced changes. However, such a mechanism needs further investigation. © 2023 The Authors

Place, publisher, year, edition, pages
Reed Elsevier India Pvt. Ltd. , 2024. Vol. 47, p. 23-27
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Surgery
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URN: urn:nbn:se:ri:diva-68103DOI: 10.1016/j.jor.2023.11.012Scopus ID: 2-s2.0-85176950867OAI: oai:DiVA.org:ri-68103DiVA, id: diva2:1817857
Funder
AFA Insurance
Note

The institution of the corresponding author (KO) has received funding from AFA Insurance , Stockholm, Sweden.

Available from: 2023-12-07 Created: 2023-12-07 Last updated: 2024-01-22Bibliographically approved

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Håkansson, JoakimRosendahl, Jennifer

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