Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Displacement of adsorbed insulin by Tween 80 monitored using total internal reflection fluorescence and ellipsometry
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, YKI – Ytkemiska institutet.ORCID iD: 0000-0002-3350-0242
2005 (English)In: Pharmaceutical research, ISSN 0724-8741, E-ISSN 1573-904X, Vol. 22, p. 1931-1941Article in journal (Refereed) Published
Abstract [en]

This study was conducted to investigate the mechanism of action in the displacement of adsorbed insulin from a hydrophobic surface by Tween 80 and of the competitive adsorption of the two species. Methods Total internal reflection fluorescence (TIRF) and ellipsometry were used as in situ methods to examine the processes taking place at hydrophobic model surfaces in the presence of insulin and Tween 80. Results TIRF studies showed that the displacement of insulin by Tween 80 could be fitted to a sigmoidal function, indicating a nucleation-dependent process. Furthermore, a linear dependence between the apparent rate constant and the logarithm of the Tween 80 concentration was found. Competitive adsorption from solution mixtures of insulin and Tween 80 indicated that insulin was adsorbed first, but subsequently displaced by the surfactant. This displacement proved also to be dependent on the concentration of Tween 80 in the mixture. Conclusions The results indicate that Tween 80 at concentrations above critical micelle concentration can be used to protect insulin against surface adsorption. The presence of a lag phase in the displacement at low surfactant concentration indicates that the mechanism of action for Tween 80 to reduce adsorption of insulin may be by competing for sites at the surface.

Place, publisher, year, edition, pages
2005. Vol. 22, p. 1931-1941
Keywords [en]
FITC, insulin, protein adsorption, protein displacement, TIRF, Tween
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27368OAI: oai:DiVA.org:ri-27368DiVA, id: diva2:1054372
Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2023-12-07Bibliographically approved

Open Access in DiVA

No full text in DiVA

Authority records

Elofsson, Ulla

Search in DiVA

By author/editor
Elofsson, Ulla
By organisation
YKI – Ytkemiska institutet
In the same journal
Pharmaceutical research
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 28 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf