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Publications (10 of 15) Show all publications
Håkansson, J., Juhlin, O., Hovannisyan, A., Rosendahl, J., Bogestål, Y. & Olmarker, K. (2024). Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study. Journal of Orthopaedics, 47, 23-27
Open this publication in new window or tab >>Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study
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2024 (English)In: Journal of Orthopaedics, ISSN 0972-978X, E-ISSN 2589-9082, Vol. 47, p. 23-27Article in journal (Refereed) Published
Abstract [en]

Purpose: It is known that contact of nucleus pulposus with the dorsal root ganglion may induce changes in nerve conduction and pain behavior. It has also been suggested that the behavioristic changes are caused by changes in voltage-gated ion channels, which in turn have been upregulated by TNF. Such upregulations have previously been shown for NaV 1.8 and NaV 1.9. In this investigation, we expanded the number of studied ion channels after the application of nucleus pulposus or TNF. Methods: Following removal of the left L4-5 fact joint, a disc puncture was performed and the dorsal root ganglion was exposed to nucleus pulposus (n = 5) and TNF (n = 5). Operated rats without disc puncture served as sham (n = 5) and 5 non-operated (naïve) rats were included. After 24 h, the DRGs were harvested and analyzed by quantitative PCR on validated pre-spotted primer plates displaying genes for 90 voltage-gated ion channels. Results: It was evident that the changes in operated animals were separate from the naïve rats. It was also apparent that gene expression changes in rats with nucleus pulposus or TNF application showed similar trends and were also separated from sham-operated animals. Conclusion: The application of nucleus pulposus and TNF onto the DRG in rats induces comparable changes in gene expression of several ion channels. Since the changes induced by TNF and NP are similar, one might also suspect that TNF mediates the NP-induced changes. However, such a mechanism needs further investigation. © 2023 The Authors

Place, publisher, year, edition, pages
Reed Elsevier India Pvt. Ltd., 2024
National Category
Surgery
Identifiers
urn:nbn:se:ri:diva-68103 (URN)10.1016/j.jor.2023.11.012 (DOI)2-s2.0-85176950867 (Scopus ID)
Funder
AFA Insurance
Note

The institution of the corresponding author (KO) has received funding from AFA Insurance , Stockholm, Sweden.

Available from: 2023-12-07 Created: 2023-12-07 Last updated: 2024-08-14Bibliographically approved
Cano, M. E., Lindgren, Å., Rosendahl, J., Johansson, J., Garcia-Martin, A., Galan, M. L., . . . Chinga Carrasco, G. (2024). Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum. International Journal of Biological Macromolecules, 267, Article ID 131229.
Open this publication in new window or tab >>Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
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2024 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 267, article id 131229Article in journal (Refereed) Published
Abstract [en]

Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum’s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures. 

Place, publisher, year, edition, pages
Elsevier B.V., 2024
Keywords
Gels; Glucose; Nucleic Acids; Oligosaccharides; Plants; Resistance; Sequences; Water; Capsicum; Cellulose; Gene Expression Regulation, Plant; Nanofibers; Oligosaccharides; Gene expression; Glucose; Kraft pulp; Nanocellulose; Nanofibers; RNA; carboxylated cellulose nanofibrils; cellulose nanofiber; glucuronic acid; oligosaccharide; unclassified drug; cellulose; nanofiber; oligosaccharide; Abiotic stress; Capsicum annuum; Cellulose nanofibrils; Characterization; Elicitor; Genes expression; Nano-cellulose; Nano-fibrils; Plant substrate; TEMPO-oxidized nanofibril; anion exchange chromatography; Article; atomic force microscopy; Capsicum annuum; chemical structure; conductometry; differential gene expression; down regulation; drug analysis; electrospray; gel hydrolysis; gene expression; harvest; high performance liquid chromatography; high throughput sequencing; hydrolysis; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; matrix-assisted laser desorption-ionization mass spectrometry; nonhuman; nuclear magnetic resonance spectroscopy; oxidation; oxidative stress; pathway analysis; plant immunity; plant seed; RNA extraction; ultraviolet visible spectrophotometry; water deprivation; chemistry; drug effect; gene expression regulation; genetics; pepper; Gels
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:ri:diva-73284 (URN)10.1016/j.ijbiomac.2024.131229 (DOI)2-s2.0-85190721953 (Scopus ID)
Funder
The Research Council of Norway, 284300
Note

The Research Council of Norway (Grant no. 284300 ), ANR (France, Grant ANR-18-SUS2-0001 ), MINECO (Spain, Grant PCI2018-093114 ) and SUSFOOD2 ERA-NET program (Grant SPAREC) are acknowledged for funding. Mirjana Filipovic, Ingebjørg Leirset, Johnny Kvakland Melbø, Kenneth Aasarød (RISE PFI) and Simon Standoft (RISE) are acknowledged for excellent laboratory work.

Available from: 2024-05-24 Created: 2024-05-24 Last updated: 2024-08-14Bibliographically approved
Buchta, C., Benka, B., Delatour, V., Faé, I., Griesmacher, A., Hellbert, K., . . . Cobbaert, C. M. (2024). Reference, calibration and referral laboratories – a look at current European provisions and beyond. Clinical Chemistry and Laboratory Medicine
Open this publication in new window or tab >>Reference, calibration and referral laboratories – a look at current European provisions and beyond
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2024 (English)In: Clinical Chemistry and Laboratory Medicine, ISSN 1434-6621, E-ISSN 1437-4331Article, review/survey (Refereed) Epub ahead of print
Abstract [en]

European Union (EU) regulations on in vitro diagnostics (IVD) and on serious cross-border threats to health provide for the establishment of European Reference Laboratories (EURLs) and their harmonization and cooperation with National Reference Laboratories (NRLs). While the EURLs under the IVD Regulation will be operational by 1 October 2024, the EURLs under the Regulation on serious cross-border threats to health will be operational by January 2025. Although NRLs may have been operating for a long time on the basis of national legislation, they should now cooperate with each other and with EURLs in a network of centers of excellence for the authorization and post-market surveillance of IVDs and for the epidemiological surveillance and control of communicable diseases. The term “reference laboratory” has long been used colloquially to refer to many kinds of laboratories, regardless of their tasks, competencies, responsibilities and designation. A literature search and analysis confirmed this by showing that a considerable proportion of scientific publications in 2024 use the term “reference laboratory” inappropriately. In order to clarify the roles and functioning of EURLs and NRLs, we have evaluated the relevant current EU provisions and compared the findings with those of reference laboratories designated by other organizations, calibration (reference) laboratories and referral laboratories, which are simply referred to as “reference laboratories”. With the forthcoming implementation of the EU regulations, at least the goals of providing safe and high-quality IVDs and adequate public health surveillance for communicable diseases appear to be achievable. 

Place, publisher, year, edition, pages
Walter de Gruyter GmbH, 2024
Keywords
authorization; calibration; communicable disease; diagnosis; epidemiological monitoring; epidemiological surveillance; European Union; human; patient referral; postmarketing surveillance; public health surveillance; review
National Category
Clinical Medicine
Identifiers
urn:nbn:se:ri:diva-76164 (URN)10.1515/cclm-2024-1066 (DOI)2-s2.0-85207632424 (Scopus ID)
Available from: 2024-11-19 Created: 2024-11-19 Last updated: 2024-11-19Bibliographically approved
Chinga Carrasco, G., Pasquier, E., Solberg, A., Leirset, I., Stevanic Srndovic, J., Rosendahl, J. & Håkansson, J. (2023). Carboxylated nanocellulose for wound healing applications – Increase of washing efficiency after chemical pre-treatment and stability of homogenized gels over 10 months. Carbohydrate Polymers, 314, Article ID 120923.
Open this publication in new window or tab >>Carboxylated nanocellulose for wound healing applications – Increase of washing efficiency after chemical pre-treatment and stability of homogenized gels over 10 months
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2023 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 314, article id 120923Article in journal (Refereed) Published
Abstract [en]

To commercialize a biomedical product as a medical device, reproducibility of production and time-stability are important parameters. Studies of reproducibility are lacking in the literature. Additionally, chemical pre-treatments of wood fibres to produce highly fibrillated cellulose nanofibrils (CNF) seem to be demanding in terms of production efficiency, being a bottleneck for industrial upscaling. In this study, we evaluated the effect of pH on the dewatering time and washing steps of 2,2,6,6-Tetramethylpiperidinyloxy (TEMPO)-mediated oxidized wood fibres when applying 3.8 mmol NaClO/g cellulose. The results indicate that the method does not affect the carboxylation of the nanocelluloses, and levels of approximately 1390 μmol/g were obtained with good reproducibility. The washing time of a Low-pH sample was reduced to 1/5 of the time required for washing a Control sample. Additionally, the stability of the CNF samples was assessed over 10 months and changes were quantified, the most pronounced were the increase of potential residual fibre aggregates, reduction of viscosity and increase of carboxylic acid content. The cytotoxicity and skin irritation potential were not affected by the detected differences between the Control and Low-pH samples. Importantly, the antibacterial effect of the carboxylated CNFs against S. aureus and P. aeruginosa was confirmed. © 2023 The Authors

Place, publisher, year, edition, pages
Elsevier Ltd, 2023
Keywords
Antibacterial, Degradation, Hydrolysis, Nanocellulose, TEMPO-oxidized fibres, Wound dressings, Chemical stability, Fibers, Gels, Nanofibers, pH, Production efficiency, Washing, Wood, 2, 2, 6, 6-tetramethylpiperidinyloxy-oxidized fiber, Antibacterials, Cellulose nanofibrils, Chemical pre-treatment, Nano-cellulose, Reproducibilities, Washing efficiency, Woodfiber, Wound healing applications, Carboxylation
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:ri:diva-64385 (URN)10.1016/j.carbpol.2023.120923 (DOI)2-s2.0-85152907526 (Scopus ID)
Note

Correspondence Address: Chinga-Carrasco, G.; RISE, Norway; email: gary.chinga.carrasco@rise-pfi.no; Funding details: Norges Forskningsråd, 309178; Funding text 1: The authors thank the Research Council of Norway for funding (OxyPol project - “Oxygenated biopolymers for biomedical applications”, grant no. 309178 ). 

Available from: 2023-05-03 Created: 2023-05-03 Last updated: 2023-11-03Bibliographically approved
Rosendahl, J., Zarna, C., Håkansson, J. & Chinga-Carrasco, G. (2023). Gene-Expression Analysis of Human Fibroblasts Affected by 3D-Printed Carboxylated Nanocellulose Constructs. Bioengineering, 10(1), Article ID 121.
Open this publication in new window or tab >>Gene-Expression Analysis of Human Fibroblasts Affected by 3D-Printed Carboxylated Nanocellulose Constructs
2023 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 10, no 1, article id 121Article in journal (Refereed) Published
Abstract [en]

Three-dimensional (3D) printing has emerged as a highly valuable tool to manufacture porous constructs. This has major advantages in, for example, tissue engineering, in which 3D scaffolds provide a microenvironment with adequate porosity for cell growth and migration as a simulation of tissue regeneration. In this study, we assessed the suitability of three cellulose nanofibrils (CNF) that were obtained through 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO)-mediated oxidation. The CNFs were obtained by applying three levels of carboxylation, i.e., 2.5, 3.8, and 6.0 mmol sodium hypochlorite (NaClO) per gram of cellulose. The CNFs exhibited different nanofibrillation levels, affecting the corresponding viscosity and 3D printability of the CNF gels (0.6 wt%). The scaffolds were manufactured by micro-extrusion and the nanomechanical properties were assessed with nanoindentation. Importantly, fibroblasts were grown on the scaffolds and the expression levels of the marker genes, which are relevant for wound healing and proliferation, were assessed in order to reveal the effect of the 3D-scaffold microenvironment of the cells. © 2023 by the authors.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
3D-printing, characterization, gene expression, nanocellulose, wound dressings
National Category
Biomaterials Science
Identifiers
urn:nbn:se:ri:diva-63992 (URN)10.3390/bioengineering10010121 (DOI)2-s2.0-85146750909 (Scopus ID)
Note

Funding details: 283895, 309178; Funding details: European Commission, EC; Funding details: Norges Forskningsråd, MNET17/NMCS-1204; Funding text 1: The authors acknowledge the European Commission and the Research Council of Norway for funding part of this work through the MANUNET III program (project no. MNET17/NMCS-1204), the MedIn project (grant no. 283895), “New functionalized medical devices for surgical interventions in the pelvic cavity” and the OxyPol project (“Oxygenated biopolymers for biomedical applications”, grant no. 309178).

Available from: 2023-02-15 Created: 2023-02-15 Last updated: 2023-05-22Bibliographically approved
Österberg, K., Bogestål, Y., Jenndahl, L., Gustafsson-Hedberg, T., Synnergren, J., Holmgren, G., . . . Håkansson, J. (2023). Personalized tissue-engineered veins - long term safety, functionality and cellular transcriptome analysis in large animals. Biomaterials Science, 11(11), 3860-3877
Open this publication in new window or tab >>Personalized tissue-engineered veins - long term safety, functionality and cellular transcriptome analysis in large animals
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2023 (English)In: Biomaterials Science, ISSN 2047-4830, E-ISSN 2047-4849, Vol. 11, no 11, p. 3860-3877Article in journal (Refereed) Published
Abstract [en]

Tissue engineering is a promising methodology to produce advanced therapy medicinal products (ATMPs). We have developed personalized tissue engineered veins (P-TEV) as an alternative to autologous or synthetic vascular grafts utilized in reconstructive vein surgery. Our hypothesis is that individualization through reconditioning of a decellularized allogenic graft with autologous blood will prime the tissue for efficient recellularization, protect the graft from thrombosis, and decrease the risk of rejection. In this study, P-TEVs were transplanted to vena cava in pig, and the analysis of three veins after six months, six veins after 12 months and one vein after 14 months showed that all P-TEVs were fully patent, and the tissue was well recellularized and revascularized. To confirm that the ATMP product had the expected characteristics one year after transplantation, gene expression profiling of cells from P-TEV and native vena cava were analyzed and compared by qPCR and sequencing. The qPCR and bioinformatics analysis confirmed that the cells from the P-TEV were highly similar to the native cells, and we therefore conclude that P-TEV is functional and safe in large animals and have high potential for use as a clinical transplant graft.

Place, publisher, year, edition, pages
NLM (Medline), 2023
Keywords
animal, endothelium cell, gene expression profiling, pig, procedures, tissue engineering, transplantation, vein, Animals, Endothelial Cells, Swine, Veins
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:ri:diva-65415 (URN)10.1039/d2bm02011d (DOI)2-s2.0-85160870522 (Scopus ID)
Note

This study was supported by Vinnova project CAMP (contract no. 2017-02130), a common call by VINNOVA and Vetenskapsrådet: Biologcal pharmaseuticals (Dnr 2017-02983),by University of Skövde under grants from the Swedish Knowledge Foundation [#2016-0330, #2020-0014] and Västra Götalandsregionen (consultant check).

Available from: 2023-06-15 Created: 2023-06-15 Last updated: 2023-12-22Bibliographically approved
Pasquier, E., Rosendahl, J., Solberg, A., Ståhlberg, A., Håkansson, J. & Chinga Carrasco, G. (2023). Polysaccharides and Structural Proteins as Components in Three-Dimensional Scaffolds for Breast Cancer Tissue Models: A Review. Bioengineering, 10(6), Article ID 682.
Open this publication in new window or tab >>Polysaccharides and Structural Proteins as Components in Three-Dimensional Scaffolds for Breast Cancer Tissue Models: A Review
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2023 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 10, no 6, article id 682Article in journal (Refereed) Published
Abstract [en]

Breast cancer is the most common cancer among women, and even though treatments are available, efficiency varies with the patients. In vitro 2D models are commonly used to develop new treatments. However, 2D models overestimate drug efficiency, which increases the failure rate in later phase III clinical trials. New model systems that allow extensive and efficient drug screening are thus required. Three-dimensional printed hydrogels containing active components for cancer cell growth are interesting candidates for the preparation of next generation cancer cell models. Macromolecules, obtained from marine- and land-based resources, can form biopolymers (polysaccharides such as alginate, chitosan, hyaluronic acid, and cellulose) and bioactive components (structural proteins such as collagen, gelatin, and silk fibroin) in hydrogels with adequate physical properties in terms of porosity, rheology, and mechanical strength. Hence, in this study attention is given to biofabrication methods and to the modification with biological macromolecules to become bioactive and, thus, optimize 3D printed structures that better mimic the cancer cell microenvironment. Ink formulations combining polysaccharides for tuning the mechanical properties and bioactive polymers for controlling cell adhesion is key to optimizing the growth of the cancer cells. © 2023 by the authors.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
3D bioprinting, biopolymers, breast cancer models, cells microenvironment
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:ri:diva-65684 (URN)10.3390/bioengineering10060682 (DOI)2-s2.0-85163723963 (Scopus ID)
Note

Correspondence Address: G. Chinga-Carrasco; RISE PFI AS, Trondheim, Høgskoleringen 6b, NO-7491, Norway; E.P.: A.S. (Amalie Solberg), and G.C.-C. thank the Research Council of Norway and bioMAT4EYE project (Grant 337610) for funding part of this work. A.S. (Anders Ståhlberg) is funded by Region Västra Götaland, Swedish Cancer Society (2022-2080), Swedish Childhood Cancer Foundation (2022-0030), Swedish Research Council (2021-01008); the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965065), Sweden’s Innovation Agency and the Sjöberg Foundation. J.H. and J.R. are funded by the Swedish Foundation for Strategic Research (FID15-0008), Sweden’s Innovation Agency (2017-03737 and 2021-04484) and Region Västra Götalandsregionen (RUN 2018-00017).

Available from: 2023-08-07 Created: 2023-08-07 Last updated: 2023-11-03Bibliographically approved
Chinga-Carrasco, G., Rosendahl, J. & Catalán, J. (2022). Nanocelluloses – Nanotoxicology, Safety Aspects and 3D Bioprinting. Advances in Experimental Medicine and Biology, 1357, 155-177
Open this publication in new window or tab >>Nanocelluloses – Nanotoxicology, Safety Aspects and 3D Bioprinting
2022 (English)In: Advances in Experimental Medicine and Biology, ISSN 0065-2598, E-ISSN 2214-8019, Vol. 1357, p. 155-177Article in journal (Refereed) Published
Abstract [en]

Nanocelluloses have good rheological properties that facilitate the extrusion of nanocellulose gels in micro-extrusion systems. It is considered a highly relevant characteristic that makes it possible to use nanocellulose as an ink component for 3D bioprinting purposes. The nanocelluloses assessed in this book chapter include wood nanocellulose (WNC), bacterial nanocellulose (BNC), and tunicate nanocellulose (TNC), which are often assumed to be non-toxic. Depending on various chemical and mechanical processes, both cellulose nanofibrils (CNF) and cellulose nanocrystals (CNC) can be obtained from the three mentioned nanocelluloses (WNC, BNC, and TNC). Pre/post-treatment processes (chemical and mechanical) cause modifications regarding surface chemistry and nano-morphology. Hence, it is essential to understand whether physicochemical properties may affect the toxicological profile of nanocelluloses. In this book chapter, we provide an overview of nanotoxicology and safety aspects associated with nanocelluloses. Relevant regulatory requirements are considered. We also discuss hazard assessment strategies based on tiered approaches for safety testing, which can be applied in the early stages of the innovation process. Ensuring the safe development of nanocellulose-based 3D bioprinting products will enable full market use of these sustainable resources throughout their life cycle.

Place, publisher, year, edition, pages
Springer, 2022
Keywords
3D printing, Bioprinting, Medical devices, Nanocellulose, Regulatory frameworks, Toxicology, cellulose, nanoparticle, chemistry, flow kinetics, three dimensional printing, Nanoparticles, Printing, Three-Dimensional, Rheology
National Category
Biological Sciences
Identifiers
urn:nbn:se:ri:diva-59339 (URN)10.1007/978-3-030-88071-2_7 (DOI)2-s2.0-85130766154 (Scopus ID)
Note

Funding details: Työsuojelurahasto, 117146; Funding details: Norges Forskningsråd, 309178; Funding text 1: The authors would like to thank Dr. Piia Taxell (Finnish Institute of Occupational Health) and the European Chemicals Agency (ECHA) for providing information on the workers’ regulations and the current status of nanocelluloses under the REACH regulation, respectively. Sarunas Petronis (RISE) is acknowledged for acquiring the SEM image with cancer cells. This paper was funded by the Finnish Work Environment Fund (grant no. 117146), Swedish foundation for strategic research and by the OxyPol project, Nano2021 program, Research Council of Norway (grant no. 309178).

Available from: 2022-06-22 Created: 2022-06-22 Last updated: 2023-05-22Bibliographically approved
Rosendahl, J., Svanström, A., Berglin, M., Petronis, S., Bogestål, Y., Stenlund, P., . . . Håkansson, J. (2021). 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System. Bioengineering, 8(7), Article ID 97.
Open this publication in new window or tab >>3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System
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2021 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 8, no 7, article id 97Article in journal (Refereed) Published
Abstract [en]

Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker CD44 and the migration markers VIM and SNAI1 in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker CD44 and migration marker VIM which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.

Keywords
nanocellulose, 3D printing, cancer, 3D cell culture, CNF, cancer stemness
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:ri:diva-55646 (URN)10.3390/bioengineering8070097 (DOI)
Available from: 2021-08-05 Created: 2021-08-05 Last updated: 2023-06-07Bibliographically approved
Svanström, A., Rosendahl, J., Salerno, S., Leiva, M., Gregersson, P., Berglin, M., . . . Landberg, G. (2021). Optimized alginate-based 3D printed scaffolds as a model of patient derived breast cancer microenvironments in drug discovery. Biomedical Materials, 16(4), Article ID 045046.
Open this publication in new window or tab >>Optimized alginate-based 3D printed scaffolds as a model of patient derived breast cancer microenvironments in drug discovery
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2021 (English)In: Biomedical Materials, ISSN 1748-6041, E-ISSN 1748-605X, Vol. 16, no 4, article id 045046Article in journal (Refereed) Published
Abstract [en]

The cancer microenvironment influences tumor progression and metastasis and is pivotal to consider when designing in vivo-like cancer models. Current preclinical testing platforms for cancer drug development are mainly limited to 2D cell culture systems that poorly mimic physiological environments and traditional, low throughput animal models. The aim of this work was to produce a tunable testing platform based on 3D printed scaffolds (3DPS) with a simple geometry that, by extracellular components and response of breast cancer reporter cells, mimics patient-derived scaffolds (PDS) of breast cancer. Here, the biocompatible polysaccharide alginate was used as base material to generate scaffolds consisting of a 3D grid containing periostin and hydroxyapatite. Breast cancer cell lines (MCF7 and MDA-MB-231) produced similar phenotypes and gene expression levels of cancer stem cell, epithelial-mesenchymal transition, differentiation and proliferation markers when cultured on 3DPS and PDS, contrasting conventional 2D cultures. Importantly, cells cultured on 3DPS and PDS showed scaffold-specific responses to cytotoxic drugs (doxorubicin and 5-fluorouracil) that were different from 2D cultured cells. In conclusion, the data presented support the use of a tunable alginate-based 3DPS as a tumor model in breast cancer drug discovery. © 2021 The Author(s).

Place, publisher, year, edition, pages
IOP Publishing Ltd, 2021
Keywords
Alginate, Animal cell culture, Biocompatibility, Diseases, Drug products, Gene expression, Hydroxyapatite, Physiological models, Scaffolds (biology), Stem cells, Tumors, Biocompatible polysaccharides, Breast cancer cells, Differentiation and proliferations, Epithelial-mesenchymal transition, Gene expression levels, Physiological environment, Pre-clinical testing, Tumor progressions, 3D printers
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:ri:diva-55478 (URN)10.1088/1748-605X/ac0451 (DOI)2-s2.0-85109424226 (Scopus ID)
Available from: 2021-08-06 Created: 2021-08-06 Last updated: 2023-06-07Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-8849-1793

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