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Publications (10 of 12) Show all publications
Labriere, C., Andersen, J., Albrigtsen, M., Hansen, J. & Svenson, J. (2019). Heterocyclic cellular lipid peroxidation inhibitors inspired by the marine antioxidant barettin. Bioorganic chemistry (Print), 84, 106-114
Open this publication in new window or tab >>Heterocyclic cellular lipid peroxidation inhibitors inspired by the marine antioxidant barettin
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2019 (English)In: Bioorganic chemistry (Print), ISSN 0045-2068, Vol. 84, p. 106-114Article in journal (Refereed) Published
Abstract [en]

The marine environment remains a rich source for the discovery and development of novel bioactive compounds. The present paper describes the design, synthesis and biological evaluation of a library of small molecule heterocyclic mimetics of the marine 2,5-diketopiperazine barettin which is a powerful natural antioxidant. By mainly focusing on the influence from the brominated indole and heterocyclic core of barettin, a library of 19 compounds was prepared. The compounds comprised a heterocyclic core, either a 2,5 diketopiperazine, an imidazolidinedione or a thioxothiazolidinone, which were mainly monosubstituted with ranging bulky substituents. The prepared compounds were screened for activity in a cellular lipid peroxidation assay using HepG2 cells. Several of the synthetic compounds showed antioxidant properties superior to the positive control barettin. Two of the prepared compounds displayed inhibitory activity similar to commercial antioxidants with significant inhibition at low µg/mL concentrations. The toxicity of the compounds was also investigated against MRC-5 lung fibroblasts and none of the included compounds displayed any toxicity at 50 µg/mL.

Keywords
2, 5-Diketopiperazine, Barettin, CLPAA, Heterocycle, Marine natural products, Structure–activity relationship
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-36590 (URN)10.1016/j.bioorg.2018.11.024 (DOI)2-s2.0-85057210847 (Scopus ID)
Note

 Funding details: VINNOVA, 2014-01435; Funding details: Norges Forskningsråd, ES508288;

Available from: 2018-12-06 Created: 2018-12-06 Last updated: 2018-12-06Bibliographically approved
Moodie, L. W. K., Sepčić, K., Turk, T., FrangeŽ, R. & Svenson, J. (2019). Natural cholinesterase inhibitors from marine organisms. Natural product reports (Print)
Open this publication in new window or tab >>Natural cholinesterase inhibitors from marine organisms
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2019 (English)In: Natural product reports (Print), ISSN 0265-0568, E-ISSN 1460-4752Article in journal (Refereed) Epub ahead of print
Abstract [en]

Covering: Published between 1974 up to 2018Inhibition of cholinesterases is a common approach for the management of several disease states. Most notably, cholinesterase inhibitors are used to alleviate the symptoms of neurological disorders like dementia and Alzheimer's disease and treat myasthenia gravis and glaucoma. Historically, most drugs of natural origin have been isolated from terrestrial sources and inhibitors of cholinesterases are no exception. However, the last 50 years have seen a rise in the quantity of marine natural products with close to 25 000 reported in the scientific literature. A number of marine natural products with potent cholinesterase inhibitory properties have also been reported; isolated from a variety of marine sources from algae to ascidians. Representing a diverse range of structural classes, these compounds provide inspirational leads that could aid the development of therapeutics. The current paper aims to, for the first time, comprehensively summarize the literature pertaining to cholinesterase inhibitors derived from marine sources, including the first papers published in 1974 up to 2018. The review does not report bioactive extracts, only isolated compounds, and a specific focus lies on compounds with reported dose-response data. In vivo and mechanistic data is included for compounds where this is reported. In total 185 marine cholinesterase inhibitors and selected analogs have been identified and reported and some of the compounds display inhibitory activities comparable or superior to cholinesterase inhibitors in clinical use.

National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-38271 (URN)10.1039/c9np00010k (DOI)30924818 (PubMedID)
Available from: 2019-04-02 Created: 2019-04-02 Last updated: 2019-07-01Bibliographically approved
Svendsen, J., Grant, T., Rennison, D., Brimble, M. & Svenson, J. (2019). Very Short and Stable Lactoferricin-Derived Antimicrobial Peptides: Design Principles and Potential Uses. Accounts of Chemical Research, 52(3), 749-759
Open this publication in new window or tab >>Very Short and Stable Lactoferricin-Derived Antimicrobial Peptides: Design Principles and Potential Uses
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2019 (English)In: Accounts of Chemical Research, ISSN 0001-4842, E-ISSN 1520-4898, Vol. 52, no 3, p. 749-759Article in journal (Refereed) Published
Abstract [en]

ConspectusThe alarming rate at which micro-organisms are developing resistance to conventional antibiotics represents one of the global challenges of our time. There is currently ample space in the antibacterial drug pipeline, and scientists are trying to find innovative and novel strategies to target the microbial enemies. Nature has remained a source of inspiration for most of the antibiotics developed and used, and the immune molecules produced by the innate defense systems, as a first line of defense, have been heralded as the next source of antibiotics. Most living organisms produce an arsenal of antimicrobial peptides (AMPs) to rapidly fend off intruding pathogens, and several different attempts have been made to transform this versatile group of compounds into the next generation of antibiotics. However, faced with the many hurdles of using peptides as drugs, the success of these defense molecules as therapeutics remains to be realized. AMPs derived from the proteolytic degradation of the innate defense protein lactoferrin have been shown to display several favorable antimicrobial properties. In an attempt to investigate the biological and pharmacological properties of these much shorter AMPs, the sequence dependence was investigated, and it was shown, through a series of truncation experiments, that these AMPs in fact can be prepared as tripeptides, with improved antimicrobial activity, via the incorporation of unnatural hydrophobic residues and terminal cappings. In this Account, we describe how this class of promising cationic tripeptides has been developed to specifically address the main challenges limiting the general use of AMPs. This has been made possible through the identification of the antibacterial pharmacophore and via the incorporation of a range of unnatural hydrophobic and cationic amino acids. Incorporation of these residues at selected positions has allowed us to extensively establish how these compounds interact with the major proteolytic enzymes trypsin and chymotrypsin and also the two major drug-binding plasma proteins serum albumin and α-1 glycoprotein. Several of the challenges associated with using AMPs relate to their size, susceptibility to rapid proteolytic degradation, and poor oral bioavailability. Our studies have addressed these issues in detail, and the results have allowed us to effectively design and prepare active and metabolically stable AMPs that have been evaluated in a range of functional settings. The optimized short AMPs display inhibitory activities against a plethora of micro-organisms at low micromolar concentrations, and they have been shown to target resistant strains of both bacteria and fungi alike with a very rapid mode of action. Our Account further describes how these compounds behave in in vivo experiments and highlights both the challenges and possibilities of the intriguing compounds. In several areas, they have been shown to exhibit comparable or superior activity to established antibacterial, antifungal, and antifouling commercial products. This illustrates their ability to effectively target and eradicate various microbes in a variety of settings ranging from the ocean to the clinic.

National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-38258 (URN)10.1021/acs.accounts.8b00624 (DOI)2-s2.0-85063158131 (Scopus ID)
Available from: 2019-04-02 Created: 2019-04-02 Last updated: 2019-04-02Bibliographically approved
Brooke, D. G., Cervin, G., Champeau, O., Harwood, D. T., Pavia, H., Selwood, A. I., . . . Cahill, P. L. (2018). Antifouling activity of portimine, select semisynthetic analogues, and other microalga-derived spirocyclic imines. Biofouling (Print), 34(8), 950-961
Open this publication in new window or tab >>Antifouling activity of portimine, select semisynthetic analogues, and other microalga-derived spirocyclic imines
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2018 (English)In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 34, no 8, p. 950-961Article in journal (Refereed) Published
Abstract [en]

A range of natural products from marine invertebrates, bacteria and fungi have been assessed as leads for nature-inspired antifouling (AF) biocides, but little attention has been paid to microalgal-derived compounds. This study assessed the AF activity of the spirocyclic imine portimine (1), which is produced by the benthic mat-forming dinoflagellate Vulcanodinium rugosum. Portimine displayed potent AF activity in a panel of four macrofouling bioassays (EC50 0.06–62.5 ng ml−1), and this activity was distinct from that of the related compounds gymnodimine-A (2), 13-desmethyl spirolide C (3), and pinnatoxin-F (4). The proposed mechanism of action for portimine is induction of apoptosis, based on the observation that portimine inhibited macrofouling organisms at developmental stages known to involve apoptotic processes. Semisynthetic modification of select portions of the portimine molecule was subsequently undertaken. Observed changes in bioactivity of the resulting semisynthetic analogues of portimine were consistent with portimine’s unprecedented 5-membered imine ring structure playing a central role in its AF activity.

Keywords
Bioassay, biofouling, microalgae, natural product, portimine
National Category
Chemical Engineering
Identifiers
urn:nbn:se:ri:diva-37308 (URN)10.1080/08927014.2018.1514461 (DOI)2-s2.0-85058480982 (Scopus ID)
Available from: 2019-01-18 Created: 2019-01-18 Last updated: 2019-03-29Bibliographically approved
Moodie, L. W. K., Cervin, G., Trepos, R., Labriere, C., Hellio, C., Pavia, H. & Svenson, J. (2018). Design and Biological Evaluation of Antifouling Dihydrostilbene Oxime Hybrids. Marine Biotechnology, 20(2), 257-267
Open this publication in new window or tab >>Design and Biological Evaluation of Antifouling Dihydrostilbene Oxime Hybrids
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2018 (English)In: Marine Biotechnology, ISSN 1436-2228, E-ISSN 1436-2236, Vol. 20, no 2, p. 257-267Article in journal (Refereed) Published
Abstract [en]

By combining the recently reported repelling natural dihydrostilbene scaffold with an oxime moiety found in many marine antifoulants, a library of nine antifouling hybrid compounds was developed and biologically evaluated. The prepared compounds were shown to display a low antifouling effect against marine bacteria but a high potency against the attachment and growth of microalgae down to MIC values of 0.01 μg/mL for the most potent hybrid. The mode of action can be characterized as repelling via a reversible non-toxic biostatic mechanism. Barnacle cyprid larval settlement was also inhibited at low μg/mL concentrations with low levels or no toxicity observed. Several of the prepared compounds performed better than many reported antifouling marine natural products. While several of the prepared compounds are highly active as antifoulants, no apparent synergy is observed by incorporating the oxime functionality into the dihydrostilbene scaffold. This observation is discussed in light of recently reported literature data on related marine natural antifoulants and antifouling hybrids as a potentially general strategy for generation of improved antifoulants. 

Keywords
Antifouling, Batatasin, Dihydrostilbene, Hybrid, Ianthelline, Oxime
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-33508 (URN)10.1007/s10126-018-9802-z (DOI)2-s2.0-85043472294 (Scopus ID)
Available from: 2018-03-23 Created: 2018-03-23 Last updated: 2018-12-12Bibliographically approved
Labrière, C., Kondori, N., Caous, J. S., Boomgaren, M., Sandholm, K., Ekdahl, K. N. N., . . . Svenson, J. (2018). Development and evaluation of cationic amphiphilic antimicrobial 2,5-diketopiperazines. Journal of Peptide Science, 24(7), Article ID e3090.
Open this publication in new window or tab >>Development and evaluation of cationic amphiphilic antimicrobial 2,5-diketopiperazines
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2018 (English)In: Journal of Peptide Science, ISSN 1075-2617, E-ISSN 1099-1387, Vol. 24, no 7, article id e3090Article in journal (Refereed) Published
Abstract [en]

Both pathogenic bacteria and fungi are developing resistance to common antimicrobial treatment at an alarming rate. To counteract this development, it is of essence to develop new classes of antimicrobial agents. One such class is antimicrobial peptides, most of which are derived from the innate immune system. In this study, a series of novel 2,5-diketopiperazines were designed, synthesized, and evaluated for their antimicrobial abilities. The compounds were designed to probe the pharmacophore dictated for short linear mimics of antimicrobial cationic peptides, and as such, the compounds contain a range of cationic and hydrophobic functionalities. Several of the prepared compounds displayed high antimicrobial activities toward bacteria and also against human pathogenic fungi. Of particular interest was the high activity toward fungal strains with an inherent increased resistance toward conventional antifungal agents. The most effective compounds displayed inhibition of Candida glabrata and Candida krusei growth at concentrations between 4 and 8 μg/mL, which is comparable to commercial antifungal agents in use. Structure activity relationship studies revealed a similar dependence on cationic charge and the volume of the hydrophobic bulk as for linear cationic antimicrobial peptides. Finally, the hemolytic activity of selected compounds was evaluated, which revealed a potential to produce active compounds with attenuation of unwanted hemolysis. The findings highlight the potential of cyclic cationic amphiphilic peptidomimetics as a class of promising compounds for the treatment of infections caused by microorganisms with an increased resistance to conventional antimicrobial agents. © 2018 European Peptide Society and John Wiley & Sons, Ltd.

Place, publisher, year, edition, pages
John Wiley and Sons Ltd, 2018
Keywords
2, 5-diketopiperazine, Antifungal agents, Antimicrobial, Candida krusei, MRSA, Structure-activity relationship
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-34356 (URN)10.1002/psc.3090 (DOI)2-s2.0-85047664224 (Scopus ID)
Available from: 2018-08-07 Created: 2018-08-07 Last updated: 2019-01-16Bibliographically approved
Olsen, E. K., Hansen, E., Moodie, L. W. K., Isaksson, J., Sepčić, K., Cergolj, M., . . . Andersen, J. H. (2016). Marine AChE inhibitors isolated from Geodia barretti: Natural compounds and their synthetic analogs. Organic and biomolecular chemistry, 14(5), 1629-1640
Open this publication in new window or tab >>Marine AChE inhibitors isolated from Geodia barretti: Natural compounds and their synthetic analogs
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2016 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 14, no 5, p. 1629-1640Article in journal (Refereed) Published
Abstract [en]

Barettin, 8,9-dihydrobarettin, bromoconicamin and a novel brominated marine indole were isolated from the boreal sponge Geodia barretti collected off the Norwegian coast. The compounds were evaluated as inhibitors of electric eel acetylcholinesterase. Barettin and 8,9-dihydrobarettin displayed significant inhibition of the enzyme, with inhibition constants (Ki) of 29 and 19 μM respectively towards acetylcholinesterase via a reversible noncompetitive mechanism. These activities are comparable to those of several other natural acetylcholinesterase inhibitors of marine origin. Bromoconicamin was less potent against acetylcholinesterase, and the novel compound was inactive. Based on the inhibitory activity, a library of 22 simplified synthetic analogs was designed and prepared to probe the role of the brominated indole, common to all the isolated compounds. From the structure-activity investigation it was shown that the brominated indole motif is not sufficient to generate a high acetylcholinesterase inhibitory activity, even when combined with natural cationic ligands for the acetylcholinesterase active site. The four natural compounds were also analysed for their butyrylcholinesterase inhibitory activity in addition and shown to display comparable activities. The study illustrates how both barettin and 8,9-dihydrobarettin display additional bioactivities which may help to explain their biological role in the producing organism. The findings also provide new insights into the structure-activity relationship of both natural and synthetic acetylcholinesterase inhibitors.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2016
National Category
Medicinal Chemistry Biochemistry and Molecular Biology Biocatalysis and Enzyme Technology Organic Chemistry
Identifiers
urn:nbn:se:ri:diva-95 (URN)10.1039/c5ob02416a (DOI)2-s2.0-84956629668 (Scopus ID)
Available from: 2016-05-31 Created: 2016-04-28 Last updated: 2019-06-17Bibliographically approved
Solstad, R. G., Li, C., Isaksson, J., Johansen, J., Svenson, J., Stensvåg, K. & Haug, T. (2016). Novel antimicrobial peptides EeCentrocins 1, 2 and EeStrongylocin 2 from the Edible sea urchin Echinus esculentus have 6-br-trp post-translational modifications. PLoS ONE, 11(3), Article ID e0151820.
Open this publication in new window or tab >>Novel antimicrobial peptides EeCentrocins 1, 2 and EeStrongylocin 2 from the Edible sea urchin Echinus esculentus have 6-br-trp post-translational modifications
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2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 3, article id e0151820Article in journal (Refereed) Published
Abstract [en]

The global problem of microbial resistance to antibiotics has resulted in an urgent need to develop new antimicrobial agents. Natural antimicrobial peptides are considered promising candidates for drug development. Echinoderms, which rely on innate immunity factors in the defence against harmful microorganisms, are sources of novel antimicrobial peptides. This study aimed to isolate and characterise antimicrobial peptides from the Edible sea urchin Echinus esculentus. Using bioassay-guided purification and cDNA cloning, three antimicrobial peptides were characterised from the haemocytes of the sea urchin; two heterodimeric peptides and a cysteine-rich peptide. The peptides were named EeCentrocin 1 and 2 and EeStrongylocin 2, respectively, due to their apparent homology to the published centrocins and strongylocins isolated from the green sea urchin Strongylocentrotus droebachiensis. The two centrocin-like peptides EeCentrocin 1 and 2 are intramolecularly connected via a disulphide bond to form a heterodimeric structure, containing a cationic heavy chain of 30 and 32 amino acids and a light chain of 13 amino acids. Additionally, the light chain of EeCentrocin 2 seems to be N-terminally blocked by a pyroglutamic acid residue. The heavy chains of EeCentrocins 1 and 2 were synthesised and shown to be responsible for the antimicrobial activity of the natural peptides. EeStrongylocin 2 contains 6 cysteines engaged in 3 disulphide bonds. A fourth peptide (Ee4635) was also discovered but not fully characterised. Using mass spectrometric and NMR analyses, EeCentrocins 1 and 2, EeStrongylocin 2 and Ee4635 were all shown to contain post-translationally brominated Trp residues in the 6 position of the indole ring.

Place, publisher, year, edition, pages
Public Library of Science, 2016
National Category
Medicinal Chemistry Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:ri:diva-99 (URN)10.1371/journal.pone.0151820 (DOI)2-s2.0-84962109454 (Scopus ID)
Available from: 2016-05-31 Created: 2016-04-28 Last updated: 2019-06-17Bibliographically approved
Svenson, J. (2016). På jakt efter nya läkemedel i Ishavet. Naturvetare, 6, 24-28
Open this publication in new window or tab >>På jakt efter nya läkemedel i Ishavet
2016 (Swedish)In: Naturvetare, ISSN 2000-2424, Vol. 6, p. 24-28Article in journal (Other (popular science, discussion, etc.)) Published
Abstract [sv]

Ungefär en tredjedel av alla läkemedel har sin källa i naturen. Forskaren Johan Svenson skriver själv om sin expedition i Arktis, där forskarna samlar in alger och andra organismer i sökandet efter nya molekyler, som till exempel kan bli ny antibiotika.

Place, publisher, year, edition, pages
Naturvetarna, 2016
National Category
Pharmaceutical Sciences Medicinal Chemistry Medical Engineering
Identifiers
urn:nbn:se:ri:diva-27775 (URN)
Available from: 2017-01-07 Created: 2017-01-07 Last updated: 2019-06-18Bibliographically approved
Stensen, W., Turner, R., Brown, M., Kondori, N., Svendsen, J. S. & Svenson, J. (2016). Short cationic antimicrobial peptides display superior antifungal activities toward Candidiasis and Onychomycosis in comparison with Terbinafine and Amorolfine. Molecular Pharmaceutics, 13(10), 3595-3600
Open this publication in new window or tab >>Short cationic antimicrobial peptides display superior antifungal activities toward Candidiasis and Onychomycosis in comparison with Terbinafine and Amorolfine
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2016 (English)In: Molecular Pharmaceutics, ISSN 1543-8384, E-ISSN 1543-8392, Vol. 13, no 10, p. 3595-3600Article in journal (Refereed) Published
Abstract [en]

Novel antifungals are in high demand due to the challenges associated with resistant, persistent, and systemic fungal infections. Synthetic mimics of antimicrobial peptides are emerging as a promising class of compounds for antifungal treatment. In the current study, five synthetic cationic antimicrobial tripeptides were evaluated as antifungal therapeutics against 24 pathogenic strains of fungi. Three of the peptides displayed strong general antifungal properties at low micromolar inhibitory concentrations. The most promising peptide, compound 5, was selected and evaluated as an antifungal remedy for Candida albicans candidiasis in a human skin model and for the treatment of Trichophyton rubrum induced onychomycosis in an infected human nail model. Compound 5 was shown to display antifungal properties and a rapid mode of action superior to those of both the commercial comparators Loceryl and Lamisil. Compound 5 was also active against a clinical isolate of Candida albicans with acquired fluconazole resistance.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2016
Keywords
onychomycosis, candidiasis, antimicrobial peptides, terbinafine, amorolfine, antifungal
National Category
Pharmaceutical Sciences Infectious Medicine Microbiology in the medical area
Identifiers
urn:nbn:se:ri:diva-13619 (URN)10.1021/acs.molpharmaceut.6b00654 (DOI)
Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2019-06-18Bibliographically approved
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-4729-9359

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