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2022 (English)In: Marine Drugs, E-ISSN 1660-3397, Vol. 20, no 7, article id 424Article in journal (Refereed) Published
Abstract [en]
A local strain of Nannochloropsis granulata (Ng) has been reported as the most productive microalgal strain in terms of both biomass yield and lipid content when cultivated in photobioreactors that simulate the light and temperature conditions during the summer on the west coast of Sweden. To further increase the biomass and the biotechnological potential of this strain in these conditions, mixotrophic growth (i.e., the simultaneous use of photosynthesis and respiration) with glycerol as an external carbon source was investigated in this study and compared with phototrophic growth that made use of air enriched with 1–2% CO2 . The addition of either glycerol or CO2-enriched air stimulated the growth of Ng and theproduction of high-value long-chain polyunsaturated fatty acids (EPA) as well as the carotenoid canthaxanthin. Bioassays in human prostate cell lines indicated the highest antitumoral activity for Ng extracts and fractions from mixotrophic conditions. Metabolomics detected betaine lipids specifically in the bioactive fractions, suggesting their involvement in the observed antitumoral effect. Genes related to autophagy were found to be upregulated by the most bioactive fraction, suggesting a possible therapeutic target against prostate cancer progression. Taken together, our results suggest that the local Ng strain can be cultivated mixotrophically in summer conditions on the west coast of Sweden for the production of high-value biomass containing antiproliferative compounds, carotenoids, and EPA. © 2022 by the authors.
Place, publisher, year, edition, pages
MDPI, 2022
Keywords
antitumoral activity, autophagy, bioassay, carotenoids, cell death pathway, CHN analysis, metabolomics, mixotrophy, Nannochloropsis, photobioreactors, polyunsaturated fatty acids
National Category
Nano Technology
Identifiers
urn:nbn:se:ri:diva-59829 (URN)10.3390/md20070424 (DOI)2-s2.0-85133428544 (Scopus ID)
Note
Funding details: Horizon 2020 Framework Programme, H2020, 844909; Funding details: Carl Tryggers Stiftelse för Vetenskaplig Forskning, CTS 20:406; Funding text 1: Funding: This research was funded by supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 844909. KMS was a recipient of a postdoctoral fellowship from the Carl Tryggers Foundation CTS 20:406.
2022-08-032022-08-032024-07-04Bibliographically approved