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Publications (10 of 18) Show all publications
Gilljam, K. M., Stenlund, P., Standoft, S., Andersen, S. B., Kaaber, K., Lund, H. & Bryn, K. R. (2023). Alginate and nanocellulose dressings with extract from salmon roe reduce inflammation and accelerate healing of porcine burn wounds.. Journal of Burn Care & Research
Open this publication in new window or tab >>Alginate and nanocellulose dressings with extract from salmon roe reduce inflammation and accelerate healing of porcine burn wounds.
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2023 (English)In: Journal of Burn Care & Research, ISSN 1559-047X, E-ISSN 1559-0488Article in journal (Refereed) Epub ahead of print
Abstract [en]

Partial thickness thermal burn wounds are characterized by prolonged inflammatory response, oxidative stress, tissue damage, and secondary necrosis. An optimal dressing for burn wounds would reduce inflammation and oxidative stress while providing a moist, absorbent, and protective cover. We have developed an extract from unfertilized salmon roe containing components with potential anti-inflammatory and antioxidative properties, called HTX. HTX has been combined with alginate from brown algae and nanocellulose from tunicates, and 3D printed into a solid hydrogel wound dressing called Collex. Here, Collex was tested on partial thickness burn wounds in Göttingen minipigs compared to Jelonet, and a variant of Collex without HTX. We found that dermal treatment of burn wounds with Collex resulted in accelerated healing at a majority of measured points over 23 days, compared to treatment with Jelonet. In comparison to Collex without HTX, Collex enhanced healing in the first week after trauma where wound progression was pronounced. Notably, Collex reduced the inflammatory response in the early post-injury phase. The anti-inflammatory response of Collex was investigated in more detail on activated M1 macrophages. We found that Collex, as well as HTX alone, significantly reduced secretion of pro-inflammatory interleukin-1β as well as intracellular levels of oxidative stress. The results from this study indicate that Collex is a potent dressing for treatment of burn wounds, with the anti-inflammatory effect of HTX beneficial in the initial phase, and the moist qualities of the hydrogel favorable both in the initial and the proceeding proliferative phase of wound healing.

National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-66538 (URN)10.1093/jbcr/irad006 (DOI)
Note

Funding: This work has been supported by ERA-NET Cofound on the Blue Bioeconomy (ID: 151), Innovation Fund Denmark, The Research Council of Norway, and Formas Sweden.

Available from: 2023-09-05 Created: 2023-09-05 Last updated: 2023-09-05Bibliographically approved
Stenlund, P., Enstedt, L., Gilljam, K., Standoft, S., Ahlinder, A., Lundin Johnson, M., . . . Berglin, M. (2023). Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds. Polymers, 15(12), Article ID 2627.
Open this publication in new window or tab >>Development of an All-Marine 3D Printed Bioactive Hydrogel Dressing for Treatment of Hard-to-Heal Wounds
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2023 (English)In: Polymers, E-ISSN 2073-4360, Vol. 15, no 12, article id 2627Article in journal (Refereed) Published
Abstract [en]

Current standard wound care involves dressings that provide moisture and protection; however, dressings providing active healing are still scarce and expensive. We aimed to develop an ecologically sustainable 3D printed bioactive hydrogel-based topical wound dressing targeting healing of hard-to-heal wounds, such as chronic or burn wounds, which are low on exudate. To this end, we developed a formulation composed of renewable marine components; purified extract from unfertilized salmon roe (heat-treated X, HTX), alginate from brown seaweed, and nanocellulose from tunicates. HTX is believed to facilitate the wound healing process. The components were successfully formulated into a 3D printable ink that was used to create a hydrogel lattice structure. The 3D printed hydrogel showed a HTX release profile enhancing pro-collagen I alpha 1 production in cell culture with potential of promoting wound closure rates. The dressing has recently been tested on burn wounds in Göttingen minipigs and shows accelerated wound closure and reduced inflammation. This paper describes the dressings development, mechanical properties, bioactivity, and safety. 

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
3D printed scaffolds, alginate, biomaterial, hard-to-heal wounds, nanocellulose, salmon roe, wound dressing, 3D printing, Biomechanics, Cell culture, Hydrogels, %moisture, 'current, 3d printed scaffold, Bioactive hydrogels, Hard-to-heal wound, Hydrogels dressings, Nano-cellulose, Wound closure, Wound dressings
National Category
Biomaterials Science
Identifiers
urn:nbn:se:ri:diva-65731 (URN)10.3390/polym15122627 (DOI)2-s2.0-85163772755 (Scopus ID)
Note

Correspondence Address: P. Stenlund; Department of Methodology, Textile and Medical Technology, RISE Research Institutes of Sweden AB, Gothenburg, Arvid Wallgrens backe 20, SE-413 46, Sweden;  This research was funded by ERA-Net Cofund on the Blue Bioeconomy—Unlocking the Potential of Aquatic Bioresources (BlueBio ID: 151), Swedish Research Council for Environment Agricultural Sciences and Spatial Planning (2019-02350), and Norwegian Research Council (311702).

Available from: 2023-08-08 Created: 2023-08-08 Last updated: 2024-01-17Bibliographically approved
Kjellin, P., Danielsson, K., Håkansson, J., Agrenius, K., Andersson, T. & Stenlund, P. (2022). Biomechanical and histomorphometric evaluation of skin integration on titanium and PEEK implants with different surface treatments. Journal of materials science. Materials in medicine, 33(10), Article ID 68.
Open this publication in new window or tab >>Biomechanical and histomorphometric evaluation of skin integration on titanium and PEEK implants with different surface treatments
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2022 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 33, no 10, article id 68Article in journal (Refereed) Published
Abstract [en]

Percutaneous implants are frequently affected by bacterial growth at the skin-implant interface. Integration between implant and surrounding skin is important to prevent bacteria from spreading to the underlying tissue. The standard method to evaluate skin-implant integration is by histomorphometry on samples which have been placed in tissue grown in vivo or ex vivo. In this study, a biomechanical method was developed and evaluated. The integration of implants into porcine skin was studied in an ex vivo model, where pig skin samples were cultivated in a nutrient solution. Cylindrical shaped implants, consisting of polyether ether ketone (PEEK) and titanium (Ti) with different surface treatments, were implanted in the skin tissue and the skin was grown in nutrient solution for 2 weeks. The implants were then extracted from the implantation site and the mechanical force during extraction was measured as a quantitative assessment of skin-implant integration. Implants from each group were also processed for histomorphometry and the degree of epidermal downgrowth (ED) and tissue to implant contact (TIC) was measured. A higher mean pullout force was observed for the PEEK implants compared to the Ti implants. Applying nanosized hydroxyapatite (HA) on Ti and PEEK increased the pullout force compared to uncoated controls, 24% for machined and 70% for blasted Ti, and 51% for machined PEEK. Treatment of Ti and PEEK with nanosized zirconium phosphate (ZrP) did not increase the pullout force. The histomorphometry analysis showed correlation between ED and pullout force, where the pullout force was inversely proportional to ED. For TIC, no significant differences were observed between the groups of same material (i.e. Ti, Ti+HA, Ti+ZrP, and PEEK, PEEK + HA, PEEK + ZrP), but it was significantly higher for PEEK compared to Ti. Scanning electron microscopy analysis was done on samples before and after the pullout tests, showing that the ZrP coating was unaffected by the 2 week ex vivo implantation and pullout procedure, no dissolution or detachment of the coating was observed. For the HA coating, a loss of coating was seen on approximately 5% of the total surface area of the implant. [Figure not available: see fulltext.] © 2022, The Author(s).

Place, publisher, year, edition, pages
Springer, 2022
Keywords
Bacteria, Biocompatibility, Biomechanics, Coatings, Ethers, Integration, Ketones, Mammals, Nutrients, Scanning electron microscopy, Tissue, Titanium carbide, Bacterial growth, Ex-vivo, Histomorphometry, Implant interfaces, In-vivo, Nutrient solution, Pullout force, Skin implants, Titania, Zirconium phosphate, Hydroxyapatite, nanohydroxyapatite, nanomaterial, polyetheretherketone, titanium, unclassified drug, benzophenone derivative, ether derivative, ketone, macrogol, polymer, animal experiment, animal tissue, Article, bone tissue, coating (procedure), controlled study, dissolution, ex vivo study, force, implantation, morphometry, nonhuman, quantitative analysis, surface area, surface property, Yorkshire pig, animal, osseointegration, pig, tooth implant, Animals, Benzophenones, Dental Implants, Durapatite, Polyethylene Glycols, Polymers, Surface Properties, Swine
National Category
Other Medical Biotechnology
Identifiers
urn:nbn:se:ri:diva-61212 (URN)10.1007/s10856-022-06687-y (DOI)2-s2.0-85139239686 (Scopus ID)
Note

Funding details: 214487; Funding details: Sahlgrenska Akademin; Funding text 1: Toponova AB, Halmstad, Sweden, is acknowledged for the interferometry measurements. Petra Hammarström Johansson at the Institute of Odontology, Sahlgrenska Academy, Gothenburg, is acknowledged for the histology processing. The study was partly financed by Region Västra Götaland FoU-card advanced (Dnr 214487); Funding text 2: Toponova AB, Halmstad, Sweden, is acknowledged for the interferometry measurements. Petra Hammarström Johansson at the Institute of Odontology, Sahlgrenska Academy, Gothenburg, is acknowledged for the histology processing. The study was partly financed by Region Västra Götaland FoU-card advanced (Dnr 214487)

Available from: 2022-12-02 Created: 2022-12-02 Last updated: 2024-01-02Bibliographically approved
Védie, E., Barry-Martinet, R., Senez, V., Berglin, M., Stenlund, P., Brisset, H., . . . Briand, J.-F. (2022). Influence of Sharklet-Inspired Micropatterned Polymers on Spatio-Temporal Variations of Marine Biofouling. Macromolecular Bioscience, 22(11), Article ID 2200304.
Open this publication in new window or tab >>Influence of Sharklet-Inspired Micropatterned Polymers on Spatio-Temporal Variations of Marine Biofouling
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2022 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 22, no 11, article id 2200304Article in journal (Refereed) Published
Abstract [en]

This article aims to show the influence of surface characteristics (microtopography, chemistry, mechanical properties) and seawater parameters on the settlement of marine micro- and macroorganisms. Polymers with nine microtopographies, three distinct mechanical properties, and wetting characteristics are immersed for one month into two contrasting coastal sites (Toulon and Kristineberg Center) and seasons (Winter and Summer). Influence of microtopography and chemistry on wetting is assessed through static contact angle and captive air bubble measurements over 3-weeks immersion in artificial seawater. Microscopic analysis, quantitative flow cytometry, metabarcoding based on the ribulose biphosphate carboxylase (rbcL) gene amplification, and sequencing are performed to characterize the settled microorganisms. Quantification of macrofoulers is done by evaluating the surface coverage and the type of organism. It is found that for long static in situ immersion, mechanical properties and non-evolutive wettability have no major influence on both abundance and diversity of biofouling assemblages, regardless of the type of organisms. The apparent contradiction with previous results, based on model organisms, may be due to the huge diversity of marine environments, both in terms of taxa and their size. Evolutive wetting properties with wetting switching back and forth over time have shown to strongly reduce the colonization by macrofoulers. © 2022 The Authors. 

Place, publisher, year, edition, pages
John Wiley and Sons Inc, 2022
Keywords
marine biofouling, metabarcoding, microtextured polymers, microtopography, wetting, Biology, Contact angle, Gene expression, Seawater, Coastal sites, Marine microorganism, Micro topography, Micropatterned, Microtextured polymer, Spatio-temporal variation, Surface characteristics, Wetting characteristics, Biofouling, polymer, chemistry, prevention and control, surface property, wettability, Polymers, Surface Properties
National Category
Engineering and Technology
Identifiers
urn:nbn:se:ri:diva-61201 (URN)10.1002/mabi.202200304 (DOI)2-s2.0-85139936889 (Scopus ID)
Note

 Funding details: Aix-Marseille Université, AMU; Funding text 1: E.V. and R.B-M. contributed equally to this work. Authors thank the IFREMER of La Seyne-sur-mer on the south coast of France and the Kristineberg Center for Marine Research and Innovation on the west coast of Sweden for the access to protected field immersion area. Authors thank the PRECYM (Aix-Marseille Université) for the characterization of the procaryote densities. Authors also thank the IEMN (Lille, France) for the access to a clean room and the formation to photolithography. This work was supported by the Région SUD- Provence-Alpes- Côte d'Azur (E. V., Ph.D. grant).; Funding text 2: E.V. and R.B‐M. contributed equally to this work. Authors thank the IFREMER of La Seyne‐sur‐mer on the south coast of France and the Kristineberg Center for Marine Research and Innovation on the west coast of Sweden for the access to protected field immersion area. Authors thank the PRECYM (Aix‐Marseille Université) for the characterization of the procaryote densities. Authors also thank the IEMN (Lille, France) for the access to a clean room and the formation to photolithography. This work was supported by the Région SUD‐ Provence‐Alpes‐ Côte d'Azur (E. V., Ph.D. grant).

Available from: 2022-12-06 Created: 2022-12-06 Last updated: 2023-06-02Bibliographically approved
Jenndahl, L., Österberg, K., Bogestål, Y., Simsa, R., Gustafsson-Hedberg, T., Stenlund, P., . . . Håkansson, J. (2022). Personalized tissue-engineered arteries as vascular graft transplants: A safety study in sheep. Regenerative Therapy, 21, 331-341
Open this publication in new window or tab >>Personalized tissue-engineered arteries as vascular graft transplants: A safety study in sheep
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2022 (English)In: Regenerative Therapy, ISSN 2352-3204, Vol. 21, p. 331-341Article in journal (Refereed) Published
Abstract [en]

Patients with cardiovascular disease often need replacement or bypass of a diseased blood vessel. With disadvantages of both autologous blood vessels and synthetic grafts, tissue engineering is emerging as a promising alternative of advanced therapy medicinal products for individualized blood vessels. By reconditioning of a decellularized blood vessel with the recipient's own peripheral blood, we have been able to prevent rejection without using immunosuppressants and prime grafts for efficient recellularization in vivo. Recently, decellularized veins reconditioned with autologous peripheral blood were shown to be safe and functional in a porcine in vivo study as a potential alternative for vein grafting. In this study, personalized tissue engineered arteries (P-TEA) were developed using the same methodology and evaluated for safety in a sheep in vivo model of carotid artery transplantation. Five personalized arteries were transplanted to carotid arteries and analyzed for safety and patency as well as with histology after four months in vivo. All grafts were fully patent without any occlusion or stenosis. The tissue was well cellularized with a continuous endothelial cell layer covering the luminal surface, revascularized adventitia with capillaries and no sign of rejection or infection. In summary, the results indicate that P-TEA is safe to use and has potential as clinical grafts. 

Place, publisher, year, edition, pages
Japanese Society of Regenerative Medicine, 2022
Keywords
ATMP, Blood vessels, Recellularization, Regenerative medicine, Scaffold, Tissue engineering, amoxicillin, attane, buprenorphine, carprofen, clopidogrel, dalteparin, dexmedetomidine, heparin, isoflurane, norocarp, poliglecaprone, promea, propofol, receptor type tyrosine protein phosphatase C, vetergesic, vetrimoxin, adventitia, anatomical concepts, angiography, animal experiment, animal model, animal tissue, antibiotic prophylaxis, arterial stiffness, artery anastomosis, artery transplantation, Article, biomechanics, biopsy technique, blood flow, blood vessel graft, blood vessel parameters, blood vessel wall, burst pressure, capillary, carotid artery transplantation, confocal laser scanning microscopy, controlled study, decellularization, decellularized blood vessel, device safety, DNA extraction, end to end anastomosis, endothelial cell layer, evening dosage, extracellular matrix, failure strain, female, fluorescence microscopy, full luminal endothelialization, histology, hyperplasia, immunohistochemistry, intimal hyperplasia, luminal surface, medical procedures, morning dosage, nonhuman, reconditioning of vascular graft, revascularization, scanning electron microscopy
National Category
Clinical Medicine
Identifiers
urn:nbn:se:ri:diva-60256 (URN)10.1016/j.reth.2022.08.005 (DOI)2-s2.0-85137671619 (Scopus ID)
Note

Funding details: Horizon 2020 Framework Programme, H2020; Funding details: H2020 Marie Skłodowska-Curie Actions, MSCA, 722779; Funding details: VINNOVA, Dnr 2017-01413, Dnr 2017–02983; Funding text 1: We want to acknowledge the staff at the Department of Experimental Biomedicine at Gothenburg University. This study was partly performed by funding from VINNOVA (Dnr 2017–02983 and Dnr 2017-01413) and Region Västra Götalandsregionen (consultant check) as well as from the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 722779 conducted within the “Training 4 Cell Regenerative Medicine” (T4CRM) network. The company VERIGRAFT AB holds a patent on peripheral whole blood perfusion of decellularized tissues and did also finance the project.; Funding text 2: We want to acknowledge the staff at the Department of Experimental Biomedicine at Gothenburg University. This study was partly performed by funding from VINNOVA ( Dnr 2017–02983 and Dnr 2017-01413 ) and Region Västra Götalandsregionen (consultant check) as well as from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 722779 conducted within the “Training 4 Cell Regenerative Medicine” (T4CRM) network. The company VERIGRAFT AB holds a patent on peripheral whole blood perfusion of decellularized tissues and did also finance the project.

Available from: 2022-10-10 Created: 2022-10-10 Last updated: 2023-10-05Bibliographically approved
Rosendahl, J., Svanström, A., Berglin, M., Petronis, S., Bogestål, Y., Stenlund, P., . . . Håkansson, J. (2021). 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System. Bioengineering, 8(7), Article ID 97.
Open this publication in new window or tab >>3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System
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2021 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 8, no 7, article id 97Article in journal (Refereed) Published
Abstract [en]

Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker CD44 and the migration markers VIM and SNAI1 in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker CD44 and migration marker VIM which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.

Keywords
nanocellulose, 3D printing, cancer, 3D cell culture, CNF, cancer stemness
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:ri:diva-55646 (URN)10.3390/bioengineering8070097 (DOI)
Available from: 2021-08-05 Created: 2021-08-05 Last updated: 2023-06-07Bibliographically approved
Kieseritzky, J., Alfort, H., Granskog, V., Hutchinson, D., Stenlund, P., Bogestål, Y., . . . Malkoch, M. (2020). DendroPrime as an adhesion barrier on fracture fixation plates: an experimental study in rabbits. Journal of Hand Surgery, European Volume, 45(7), 742-747
Open this publication in new window or tab >>DendroPrime as an adhesion barrier on fracture fixation plates: an experimental study in rabbits
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2020 (English)In: Journal of Hand Surgery, European Volume, ISSN 1753-1934, E-ISSN 2043-6289, Vol. 45, no 7, p. 742-747Article in journal (Refereed) Published
Abstract [en]

We tested the anti-adhesional effect of a new thiol-ene-based coating in a rabbit model. In 12 New Zealand white rabbits, the periosteum and cortex of the proximal phalanx of the second toe of both hind paws was scratched. Stainless steel plates were fixated with screws. One plate was coated with DendroPrime and the other left bare. The non-operated second toes of both hind paws of an additional four rabbits served as controls. Seven weeks after surgery, the soft tissue adhesion to the plates was evaluated macroscopically, and joint mobility was measured biomechanically. Toe joint mobility was about 20% greater and statistically significant in specimens with coated plates compared with the bare plates. Soft tissue overgrowth and, in some cases, synovitis or adhesions between the plate and the tendon were observed on all bare plates but not on any of the coated plates. We conclude that the thiol-ene-based coating can improve joint mobility by about 20%. This material has a potential to reduce adhesion around plates in fracture surgery. © The Author(s) 2020.

Place, publisher, year, edition, pages
SAGE Publications Ltd, 2020
Keywords
adhesion barrier, flexor tendon, Fracture, plate fixation, rabbit
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-45161 (URN)10.1177/1753193420932477 (DOI)2-s2.0-85086649097 (Scopus ID)
Available from: 2020-07-08 Created: 2020-07-08 Last updated: 2023-06-02Bibliographically approved
Stenlund, P., Kulbacka-Ortiz, K., Jönsson, S. & Brånemark, R. (2019). Loads on Transhumeral Amputees Using Osseointegrated Prostheses. Annals of Biomedical Engineering, 47(6), 1369-1377
Open this publication in new window or tab >>Loads on Transhumeral Amputees Using Osseointegrated Prostheses
2019 (English)In: Annals of Biomedical Engineering, ISSN 0090-6964, E-ISSN 1573-9686, Vol. 47, no 6, p. 1369-1377Article in journal (Refereed) Published
Abstract [en]

The treatment of the upper extremities is not as prevalent as that of the lower limbs, but it is nonetheless equally important. Today, there are no load data relating to upper extremity bone-anchored prosthesis users in the literature, but they are important in order to improve the rehabilitation protocol, design aspects and confidence of the user when it comes to loading the prosthesis in daily life. The aim of the present study was to investigate, in a population of eleven transhumeral amputees with osseointegrated implants, the load levels reached during specific prosthetic movements at maximum voluntary effort and during daily activities. The data showed a wide range of maximum load levels throughout the different activities. Furthermore, the data indicate that some test subjects felt apprehensive about loading the prosthesis, resulting in relatively low loads compared with the group as a whole. Within the limits of the present study, it was concluded that loading the implant system was subject specific, which resulted in large subject-to-subject variability. Moreover, some subjects exhibited uncertainty about the levels that could damage the fixation or the implant system. The study illustrates the diversity and uncertainty that exist in a population of transhumeral amputees treated with bone-anchored prostheses in terms of loading in daily life.

Keywords
Amputee, Bone-anchored, Load, OPRA, Osseointegration, Prosthesis, Transhumeral, Upper extremity, Bone, Loading, Prosthetics, Artificial limbs
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-38234 (URN)10.1007/s10439-019-02244-x (DOI)2-s2.0-85062774259 (Scopus ID)
Available from: 2019-03-27 Created: 2019-03-27 Last updated: 2023-06-02Bibliographically approved
Flys, O., Jarlemark, P., Petronis, S., Stenlund, P. & Rosen, B.-G. -. (2018). Applicability of characterization techniques on fine scale surfaces. Surface Topography: Metrology and Properties, 6(3), Article ID 034015.
Open this publication in new window or tab >>Applicability of characterization techniques on fine scale surfaces
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2018 (English)In: Surface Topography: Metrology and Properties, ISSN 2051-672X, Vol. 6, no 3, article id 034015Article in journal (Refereed) Published
Abstract [en]

In this study, several surface topographies typical for dental implants were evaluated by different measurement techniques. The samples were prepared by machine turning, wet chemical etching and electrochemical polishing of titanium discs. The measurement techniques included an atomic force microscope (AFM), coherence scanning interferometer (CSI) and a 3D stereo scanning electron microscope (SEM). The aim was to demonstrate and discuss similarities and differences in the results provided by these techniques when analyzing submicron surface topographies. The estimated surface roughness parameters were not directly comparable since the techniques had different surface spatial wavelength band limits. However, the comparison was made possible by applying a 2D power spectral density (PSD) function. Furthermore, to simplify the comparison, all measurements were characterized using the ISO 25178 standard parameters. Additionally, a Fourier transform was applied to calculate the instrument transfer function in order to investigate the behavior of CSI at different wavelength ranges. The study showed that 3D stereo SEM results agreed well with AFM measurements for the studied surfaces. Analyzed surface parameter values were in general higher when measured by CSI in comparison to both AFM and 3D stereo SEM results. In addition, the PSD analysis showed a higher power spectrum density in the lower frequency range 10-2-10-1 μm-1 for the CSI compared with the other techniques.

Keywords
3D-stereo SEM, AFM, Coherence scanning interferometer, power spectral density, surface topography, transfer function, Atomic force microscopy, Chemical polishing, Dental prostheses, Interferometers, Scanning, Scanning electron microscopy, Spectral density, Surface roughness, Topography, Transfer functions, Wet etching, 3d stereos, Characterization techniques, Measurement techniques, Power spectrum density, Scanning interferometers, Spatial wavelengths, Surface roughness parameters, Wavelength ranges, Parameter estimation
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-35683 (URN)10.1088/2051-672X/aacf5e (DOI)2-s2.0-85055559461 (Scopus ID)
Available from: 2018-11-06 Created: 2018-11-06 Last updated: 2023-06-07Bibliographically approved
Granskog, V., García-Gallego, S., von Kieseritzky, J., Rosendahl, J., Stenlund, P., Zhang, Y., . . . Malkoch, M. (2018). High-Performance Thiol–Ene Composites Unveil a New Era of Adhesives Suited for Bone Repair. Advanced Functional Materials, 28(26), Article ID 1800372.
Open this publication in new window or tab >>High-Performance Thiol–Ene Composites Unveil a New Era of Adhesives Suited for Bone Repair
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2018 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 28, no 26, article id 1800372Article in journal (Refereed) Published
Abstract [en]

The use of adhesives for fracture fixation can revolutionize the surgical procedures toward more personalized bone repairs. However, there are still no commercially available adhesive solutions mainly due to the lack of biocompatibility, poor adhesive strength, or inadequate fixation protocols. Here, a surgically realizable adhesive system capitalizing on visible light thiol–ene coupling chemistry is presented. The adhesives are carefully designed and formulated from a novel class of chemical constituents influenced by dental resin composites and self-etch primers. Validation of the adhesive strength is conducted on wet bone substrates and accomplished via fiber-reinforced adhesive patch (FRAP) methodology. The results unravel, for the first time, on the promise of a thiol–ene adhesive with an unprecedented shear bond strength of 9.0 MPa and that surpasses, by 55%, the commercially available acrylate dental adhesive system Clearfil SE Bond of 5.8 MPa. Preclinical validation of FRAPs on rat femur fracture models details good adhesion to the bone throughout the healing process, and are found biocompatible not giving rise to any inflammatory response. Remarkably, the FRAPs are found to withstand loads up to 70 N for 1000 cycles on porcine metacarpal fractures outperforming clinically used K-wires and match metal plates and screw implants.

Keywords
adhesives, biomedical applications, composites, photochemistry, polymeric materials, Biocompatibility, Composite materials, Dental composites, Dental materials, Fracture, Fracture fixation, Functional polymers, Medical applications, Photochemical reactions, Polymeric implants, Polymers, Chemical constituents, Coupling chemistry, Dental adhesive system, Dental resin composites, Inflammatory response, Shear bond strengths, Surgical procedures, Bone
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-33958 (URN)10.1002/adfm.201800372 (DOI)2-s2.0-85048981911 (Scopus ID)
Note

Funding details: KI, Karolinska Institutet; Funding details: MSCA-IF-2014-655649, MSCA, H2020 Marie Skłodowska-Curie Actions; Funding details: 2010-435; Funding details: 2014-03777; Funding details: 2012-0196, Knut och Alice Wallenbergs Stiftelse; 

Available from: 2018-07-03 Created: 2018-07-03 Last updated: 2023-06-07Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-9442-7245

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