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Löfström, CharlottaORCID iD iconorcid.org/0000-0001-6492-1245
Publications (10 of 56) Show all publications
Nygren, E., Gonzales Strömberg, L., Logenius, J., Husmark, U., Löfström, C. & Bergström, B. (2023). Potential sources of contamination on textiles and hard surfaces identified as high-touch sites near the patient environment. PLOS ONE, 18, Article ID e0287855.
Open this publication in new window or tab >>Potential sources of contamination on textiles and hard surfaces identified as high-touch sites near the patient environment
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2023 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 18, article id e0287855Article in journal (Refereed) Published
Abstract [en]

The hospital environment represents an important mediator for the transmission of healthcare-associated infections through direct and indirect hand contact with hard surfaces and textiles. In this study, bacteria on high-touch sites, including textiles and hard surfaces in two care wards in Sweden, were identified using microbiological culture methods and 16S rDNA sequencing. During a cross-sectional study, 176 high-touch hard surfaces and textiles were identified and further analysed using microbiological culture for quantification of total aerobic bacteria, Staphylococcus aureus, Clostridium difficile and Enterobacteriacae. The bacterial population structures were further analysed in 26 samples using 16S rDNA sequencing. The study showed a higher frequency of unique direct hand-textile contacts (36 per hour), compared to hard surfaces (2.2 per hour). Hard surfaces met the recommended standard of ≤ 5 CFU/cm2 for aerobic bacteria and ≤ 1 CFU/cm2 for S. aureus (53% and 35%, respectively) to a higher extent compared to textiles (19% and 30%, respectively) (P = 0.0488). The number of bacterial genera was higher on textiles than on the hard surfaces. Staphylococcus (30.4%) and Corynebacterium (10.9%) were the most representative genera for textiles and Streptococcus (13.3%) for hard surfaces. The fact that a big percentage of the textiles did not fulfil the criteria for cleanliness, combined with the higher bacterial diversity, compared to hard surfaces, are indicators that textiles were bacterial reservoirs and potential risk vectors for bacterial transmission. However, since most of the bacteria found in the study belonged to the normal flora, it was not possible to draw conclusions of textiles and hard surfaces as sources of healthcare associated infections. 2023 Nygren et al.

Place, publisher, year, edition, pages
Public Library of Science, 2023
Keywords
Bacteria, Cross-Sectional Studies, Hospitals, Humans, Staphylococcus aureus, Textiles, Touch, DNA 16S, aerobic bacterium, Article, bacterial transmission, bacterium culture, Clostridioides difficile, colony forming unit, contamination, controlled study, Corynebacterium, cross-sectional study, disease carrier, DNA sequencing, Enterobacteriaceae, healthcare associated infection, hospital, microbial community, microbial diversity, nonhuman, observational study, population structure, quantitative analysis, risk factor, surface property, Sweden, bacterium, genetics, human
National Category
Microbiology
Identifiers
urn:nbn:se:ri:diva-65729 (URN)10.1371/journal.pone.0287855 (DOI)2-s2.0-85164273329 (Scopus ID)
Note

Funding: Vinnova, Sweden’s innovation agency, grant no. 2014-00719 

Available from: 2023-08-07 Created: 2023-08-07 Last updated: 2023-08-07Bibliographically approved
Olsson, M., Löfkvist, K., Hagström, G., Eriksson, M., Albin, R., Löfström, C., . . . Nilsson, U. (2023). Ta vara på odlingens sidoströmmar – exemplet broccoli.
Open this publication in new window or tab >>Ta vara på odlingens sidoströmmar – exemplet broccoli
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2023 (Swedish)Report (Other academic)
Abstract [sv]

Detta projekt har utforskat möjligheter att använda sidoströmmar; broccoliblad och stjälk, från broccoliproduktion till nya torkade livsmedelsingredienser. Skördehantering, skördeekonomi, lämpliga processtekniker, livscykelanalys, mikrobiologisk analys och riskbedömning, analys av näringsämnen och bioaktiva ämnen, sensorik och marknadsaspekter har undersökts.

Publisher
p. 4
Series
LTV-fakultetens faktablad ; 2023:9
National Category
Food Science
Identifiers
urn:nbn:se:ri:diva-67400 (URN)
Note

Projektet är finansierat av Landsbygdsnätverket, EIP-Agri, projekt 2017-4500. EIP-Agri - Landsbygdsnätverket (landsbygdsnatverket.se)

Available from: 2023-09-25 Created: 2023-09-25 Last updated: 2023-09-25Bibliographically approved
Mateva, G., Pedersen, K., Sørensen, G., Torpdahl, M., Daskalov, H., Löfström, C. & Alexandar, I. (2021). Genetic polymorphism and antimicrobial resistance of salmonella enterica serovar enteritidis isolates from food chain sources. Comptes Rendus de l'Academie Bulgare des Sciences / Proceedings of the Bulgarian Academy of Sciences, 74(7), 977-986
Open this publication in new window or tab >>Genetic polymorphism and antimicrobial resistance of salmonella enterica serovar enteritidis isolates from food chain sources
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2021 (English)In: Comptes Rendus de l'Academie Bulgare des Sciences / Proceedings of the Bulgarian Academy of Sciences, ISSN 1310-1331, E-ISSN 2367-5535, Vol. 74, no 7, p. 977-986Article in journal (Refereed) Published
Abstract [en]

Salmonellosis is one of the most frequent food-borne infections. It is caused by infected food mainly of animal origin, although human to human transmission and numerous environmental contaminations may also be inflicted. Salmonella enterica serovar Enteritidis (S. Enteritidis) is a common pathogen worldwide. In this study 49 S. Enteritidis isolates from veterinary and food sources in Bulgaria obtained during the years 2004 to 2012 were analysed. The multiple-locus variable-number of tandem repeats analysis (MLVA) genotyping and the antimicrobial resistance (AMR) to compare the isolates were used. Results showed that isolates were divided into twenty-four MLVA and nine AMR profiles. The calculated Simpson's diversity index was 0.956 for MLVA and 0.693 to be changed for AMR, respectively. The most frequent MLVA profiles presented according to the order of the loci sequenced SENTR7 - SENTR5 - SENTR6 - SENTR4 - SE-3 were: 3-11-5-3-3 (n = 6); 2-8-9-5-3 (n = 5); 2-11-13-5-3 (n = 5); 2-11-12-6-3 (n = 4); 2-11-10-5-3 (n = 3); 3- 9-5-4-3 (n = 3). The AMR revealed that 53.1% of the isolates were resistant to one and 4.1% to ≥ 4 antimicrobials. The MLVA profiles obtained in this study were compared to the published data and they have not been isolated on a frequent basis. A partial match was found for isolates in Belgium, Thailand, China, and USA only.

Place, publisher, year, edition, pages
Academic Publishing House, 2021
Keywords
Antimicrobial resistance, MLVA, S. Enteritidis
National Category
Infectious Medicine
Identifiers
urn:nbn:se:ri:diva-55673 (URN)10.7546/CRABS.2021.07.04 (DOI)2-s2.0-85111122250 (Scopus ID)
Note

 Funding details: Ministry of Education, Youth and Science, MEYS, BG051PO001-3.3.05-0001; Funding text 1: #Corresponding author. The first author was supported by the Bulgarian Ministry of Education, Youth and Science grant BG051PO001-3.3.05-0001. 

Available from: 2021-08-09 Created: 2021-08-09 Last updated: 2021-08-09Bibliographically approved
Åkerlund, T., Zakikhany, K., Löfström, C., Lindmark, E., Källberg, H., Elofsson, U., . . . Björndal, Å. S. (2021). Stable IgG-antibody levels in patients with mild SARS-CoV-2 infection. Medrxiv
Open this publication in new window or tab >>Stable IgG-antibody levels in patients with mild SARS-CoV-2 infection
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2021 (English)In: MedrxivArticle in journal (Refereed) Published
Abstract [en]

More knowledge regarding persistence of antibody response to SARS-CoV-2 infections in the general population with mild symptoms is needed. We measured and compared levels of SARS CoV-2 spike- and nucleocapsid-specific IgG-antibodies in serum samples from 145 laboratory confirmed COVID-19 cases and 324 non-cases. The IgG-antibody levels against the spike protein in cases were stable over the time-period studied (14 to 256 days), while antibody levels against the nucleocapsid protein decreased over time

National Category
Infectious Medicine
Identifiers
urn:nbn:se:ri:diva-56949 (URN)10.1101/2021.06.16.21258960 (DOI)
Note

This project was exclusively funded by the Public Health Agency of Sweden via an assignment (S2020_05026) from the Ministry of Health and Social Affairs, Government of Sweden.

Available from: 2021-11-19 Created: 2021-11-19 Last updated: 2023-12-07Bibliographically approved
Olsson, M., Löfström, C., Bergman, P., Hagström, G., Ekman Nilsson, A., Ekblad, J., . . . Nilsson, U. (2020). Utveckling av näringsladdad broccolisoppa.
Open this publication in new window or tab >>Utveckling av näringsladdad broccolisoppa
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2020 (Swedish)Other (Other academic)
Abstract [sv]

Hälsosamma livsmedel är idag något som många konsumenter prioriterar när de väljer mat till måltider, och intresset är också stort för färdiga rätter. Detta projekt initierades för att öka intresset för konsumtion av broccoli, en produkt med bra hälsovärde beaktat innehåll av näringsämnen och andra nyttiga ämnen, och att möta konsumenters efterfrågan på hälsosamma färdiga rätter. Broccolisorten ’Beneforte’ valdes eftersom denna sort har en dokumenterad högre halt av glukorafanin*. Det pågår kliniska studier i Europa om hälsoeffekter av broccolisorter med hög halt av detta ämne och dessa skulle kunna leda till att ett hälsopåstående blir godkänt av EFSA, den europeiska livssäkerhetsmyndigheten. Produkter baserade på denna broccolisort skulle då kunna marknadsföras med hälsopåståendet.

Publisher
p. 4
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-48910 (URN)
Available from: 2020-09-25 Created: 2020-09-25 Last updated: 2023-06-05
Ottosson, J., Dryselius, R., Egervärn, M., Jacobsson, K., Svanström, Å., Forsman, M., . . . Mogren, L. (2019). Bevattningsvatten: kunskapsunderlag.
Open this publication in new window or tab >>Bevattningsvatten: kunskapsunderlag
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2019 (Swedish)Report (Other academic)
Abstract [sv]

Livsmedel från växtriket kan i vissa fall sprida mikrobiologisk smitta. Bär, bladgrönt och groddarmedför en särskild risk. Det beror på att de ofta äts råa eller efter minimal tillredning. Vi värmer deminte innan vi äter dem.Om livsmedlen är kontaminerade är det med stor sannolikhet orsakat av bevattningen. Vattnet kan havarit påverkat av avföring. Det kan också bero på spillning från vilda djur. Även naturgödsel kanförorena produkterna vid stänk från jord.Syftet med detta projekt var att se över kvaliteten på vatten för bevattning i Sverige. Vi ville koppladet till mikrobiologiska risker och utifrån det ta fram underlag till nationella råd och riktlinjer. Måletär ”en säkrare produktion av ätfärdiga bär och grönsaker som bättre förebygger sjukdomsutbrott”.Det visade sig att kvaliteten på vattnet generellt sett var god hos odlarna i projektet. Det samma gällergrödor. I vatten hittade vi enstaka sjukdomsframkallande mikroorganismer och ESBL-bildande E. coli.Dessa hittade vi dock endast i ytvatten som var mer förorenade av avföring och spillning. Men endastett fåtal prov på gröda visade på högre fekal förorening. Majoriteten av dessa kom från en och sammaodlare, som bevattnade med vatten från en damm. Vi hittade inga patogener på grödor. Däremot var ettprov på vitkål positivt för ESBL-bildande E. coli.Vi gjorde en kvantitativ riskvärdering av Shigatoxinproducerande E. coli (STEC) från isbergssallat.Den visade att även en liten förorening orsakad av avföring och utan påvisade STEC-bakterier ibevattningsvattnet kan utgöra en risk för mag-och tarminfektion. Riskvärderingen kan användas somunderlag för att föreslå mikrobiologiska kriterier, till exempel på vattenkvalitet. Vi behöver dockbeakta osäkerheterna med värderingen. De viktigaste osäkerheterna var utsöndringen av ochpatogeniciteten hos STEC från infekterade nötkreatur. Hur mycket av dessa fastnar på den bevattnadegrödan? Hur stor är sannolikheten att infekteras vid låga doser (enstaka STEC-bakterier)? Och hur storär risken av stänk från jord?Det finns olika åtgärder att ta till för att minska sannolikheten för utbrott och sjukdomsfall. Dessa är 1.Kriterier för vattenkvalitet. 2: Rening av vatten som inte uppnår dessa kriterier. 3. Införa enuppehållstid mellan sista bevattning och skörd. 4. Dessutom kan konsumenterna skölja produkternainnan de konsumerar dem. Det är också viktigt att se till att hålla utrustningen ren, exempelvis rör ochmunstycken. På så vis undviks föroreningar och tillväxt av bakterier.

Om ytvatten måste användas för bevattning under de sista två veckorna innan skörd bör ettprovtagningsprogram utformas. Proven bör då visa på låg fekal förorening samt avsaknad avSalmonella. I bästa fall bör ytvatten renas före bevattning. Detta kan göras till exempel med hjälp avfotokatalys, UV-ljus eller filtrering. De sista två dagarna före skörd bör dock endast vatten avdricksvattenkvalitet användas.I en framtid med sjunkande grundvattennivåer, torrare somrar och blötare vintrar finns ett behov av attsamla in vatten för bevattning. Det bör göras under höstregn och vårfloder, samt vintertid. Vattnet fårsedan lagras i magasin. Vatten från bevattningsmagasin kan behöva renas innan det används förbevattning beroende på hur skyddat magasinet är från föroreningar.

Abstract [en]

Vegetable products such as berries, leafy greens and sprouts pose a particular risk of transmittinginfectious diseases since they are eaten raw. Irrigation using faecally contaminated water is the mostlikely source of infectious agents but wild animal faeces and manure can also contaminate the productsfrom soil. The purpose of this project was to provide an overview of irrigation water quality inSweden, link it to microbiological risks and, based on this information, develop data for nationalguidelines with the aim of safe production of edible berries and vegetables that better prevent diseaseoutbreaks.The quality of both water and crops was generally good at the farms included in the project. Pathogensand ESBL-producing E. coli were occasionally found in water. However, these were only isolatedfrom surface water samples that were more polluted with respect to faecal indicators. Only a few cropsamples showed higher faecal contamination. The majority of these samples were from one farmerusing pond water for irrigation. No pathogens were detected on the crops. However, one cabbagesample tested positive for ESBL-producing E. coli.A quantitative risk assessment of Shiga toxin-producing E. coli (STEC) from iceberg lettuce showedthat even low faecal contamination and the absence of detected pathogens in irrigation water can stillpose a risk of gastrointestinal infection. This risk assessment can provide the basis for microbiologicalcriteria, for example, with regard to water quality. However, the uncertainties associated with the riskassessment need to be taken into account. The main uncertainties were 1: the excretion andpathogenicity of STEC from infected cattle, 2: the proportion of these that adhere to the irrigated crop,3: the likelihood of being infected at low doses (single STEC bacterium) and 4: the importance of soilcontamination.Various measures that can reduce the likelihood of foodborne outbreaks and disease cases are 1:criteria for water quality, 2: purification of water not achieving these criteria, 3: residence timebetween irrigation and harvest and 4: consumers rinsing the produce. It is also important for farmers tokeep equipment such as pipes and nozzles clean to avoid contamination and bacterial growth.

If surface water is used for irrigation (for example, in the absence of a safe groundwater supply),during the two weeks prior to harvest, a sampling programme should be implemented to ensure lowfaecal contamination and the absence of Salmonella. However, only water of drinking water qualityshould be used two days prior to harvest. It would be preferable for surface water to undergopurification before irrigation. This can be achieved, for example, by photocatalysis, UV light orfiltration.In a future with decreasing groundwater levels, drier summers and rainier winters, there is a need tocollect water for irrigation during the autumn rains, spring floods and wintertime to be stored inreservoirs for later use. Water from reservoirs may need purification before being used for irrigation,depending on how protected the reservoir is from contamination.

Publisher
p. 62
Series
LIVSMEDELSVERKETS SAMARBETSRAPPORT, ISSN 1104-7089 ; S 2019 nr 02
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-40215 (URN)
Available from: 2019-10-07 Created: 2019-10-07 Last updated: 2021-06-14Bibliographically approved
Leekitcharoenphon, P., Srensen, G., Löfström, C., Battisti, A., Szabo, I., Wasyl, D., . . . Hendriksen, R. S. (2019). Cross-Border Transmission of Salmonella Choleraesuis var. Kunzendorf in European Pigs and Wild Boar: Infection, Genetics, and Evolution. Frontiers in Microbiology, 10, Article ID 00179.
Open this publication in new window or tab >>Cross-Border Transmission of Salmonella Choleraesuis var. Kunzendorf in European Pigs and Wild Boar: Infection, Genetics, and Evolution
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2019 (English)In: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 10, article id 00179Article in journal (Refereed) Published
Abstract [en]

Salmonella enterica subspecies enterica serotype Choleraesuis is a swine adapted serovar. S. Choleraesuis variant Kunzendorf are responsible for the majority of outbreaks among pigs. S. Choleraesuis is rare in Europe, although there have been serious outbreaks in pigs including two outbreaks in Denmark in 1999-2000 and 2012-2013. Here, we elucidate the epidemiology, possible transmission routes and sources, and clonality of European S. Choleraesuis isolates including the Danish outbreak isolates. A total of 102 S. Choleraesuis isolates from different European countries and the United States of America, covering available isolates from the last two decades were selected for whole genome sequencing. We applied a temporally structured sequence analysis within a Bayesian framework to reconstruct a temporal and spatial phylogenetic tree. MLST type, resistance genes, plasmid replicons and accessory genes were identified using bioinformatics tools. Fifty-eight isolates including 11 out of 12 strains from wild boars were pan-susceptible. The remaining isolates carried multiple resistance genes. Eleven different plasmid replicons in eight plasmids were determined among the isolates. Accessory genes were associated to the identified resistance genes and plasmids. The European S. Choleraesuis was estimated to have emerged in 1837 (95% credible interval, 1733 - 1983) with the mutation rate of 1.02 SNPs/genome/year. The isolates were clustered according to countries and neighbour countries. There were transmission events between strains from the USA and European countries. Wild boar and pig isolates were genetically linked suggesting cross-border transmission and transmission due to a wildlife reservoir. The phylogenetic tree shows that multiple introductions were responsible for the outbreak of 2012-2013 in Denmark, and suggests that poorly disinfected vehicles crossing the border into Denmark were potentially the source of the outbreak. Low levels of single nucleotide polymorphisms (SNP) differences (0-4 SNPs) can be observed between clonal strains isolated from different organs of the same animal. Proper disinfection of livestock vehicles and improved quality control of livestock feed are recommended to prevent future spread of S. Choleraesuis or other more serious infectious diseases such as African swine fever (ASF) into the European pig production system.

National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-37728 (URN)10.3389/fmicb.2019.00179 (DOI)2-s2.0-85065875572 (Scopus ID)
Available from: 2019-02-06 Created: 2019-02-06 Last updated: 2024-01-17Bibliographically approved
Mateva, G., Pedersen, K., Sörensen, G., Torpdahl, M., Daskalov, H., Löfström, C. & Alexandar, I. (2019). Genetic polymorphism and antimicrobial resistance of Salmonellaenterica serovar Enteritidis isolates from veterinary and food sourcesin Bulgaria. In: : . Paper presented at European Scientific Conference on Applied Infectious Disease Epidemiology, Stockholm, Sweden, 2019.
Open this publication in new window or tab >>Genetic polymorphism and antimicrobial resistance of Salmonellaenterica serovar Enteritidis isolates from veterinary and food sourcesin Bulgaria
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2019 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Background:Salmonellosis is one of the most frequent food-borne infections. It iscaused by contaminated food mainly of animal origin, although humanto human transmission and numerous environmental contaminationsmay also be inflicted. Between 30 and 60% of the reported food-borneoutbreaks in the EU are caused by Salmonella and this is the secondmost commonly reported food-borne infection causing gastroenteritis.Salmonella enterica serovar Enteritidis (S. Enteritidis) is the mostwidespread serovar in Europe and other parts of the world.

Methods:In this study we analysed forty-nine S. Enteritidis isolates from veterinaryand food sources in Bulgaria obtained during the period of eight years.We used multiple-locus variable-number of tandem repeats analysis(MLVA) genotyping and the classical antimicrobial resistance (AMR) discdiffusion method to test the diversity of the isolates.

Results:Results showed that isolates were divided into twenty-four MLVA andnine AMR profiles. The calculated Simpsons diversity index was 0.956for MLVA and 0.754 for AMR, respectively. The AMR testing revealed that47% of the isolates were resistant to one and 4% to ≥4 antimicrobials.The most frequent resistotypes were resistance to sulphonamides (n=21)and sensitive to all compounds (n=9). The most frequent MLVA profileswere 3-5-3-3-11 (n=6); 5-13-2-3-11 (n=5); 5-9-2-3-8 (n=5); 6-12-2-3-11(n=4); 5-10-2-3-11 (n=3); 4-5-3-3-9 (n=3). MLVA profiles are presentedaccording to the order of the loci sequenced: SE1, SE2, SE9, SE3 and SE5.We searched for similar S. Enteritidis MLVA profiles in published data. Apartial match was found for some profiles only.

Conclusions:It could be concluded that the MLVA profiles of S. Enteritidis obtained inthis study (the first ones for Bulgaria) have not been frequently isolatedin other counties.

Keywords
Salmonellosis, S. Enteritidis, Zoonoses, Genetic testing
National Category
Engineering and Technology
Identifiers
urn:nbn:se:ri:diva-42458 (URN)
Conference
European Scientific Conference on Applied Infectious Disease Epidemiology, Stockholm, Sweden, 2019
Available from: 2020-01-08 Created: 2020-01-08 Last updated: 2020-01-09Bibliographically approved
Mateva, G., Pedersen, K., Sørensen, G., Asseva, G., Daskalov, H., Petrov, P., . . . Löfström, C. (2018). Use of multiple-locusvariable-numberof tandem repeatsanalysis (MLVA) to investigate genetic diversity of Salmonellaenterica subsp. enterica serovar Typhimurium isolates fromhuman, food, and veterinary sources. MicrobiologyOpen, 7(1), Article ID e00528.
Open this publication in new window or tab >>Use of multiple-locusvariable-numberof tandem repeatsanalysis (MLVA) to investigate genetic diversity of Salmonellaenterica subsp. enterica serovar Typhimurium isolates fromhuman, food, and veterinary sources
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2018 (English)In: MicrobiologyOpen, E-ISSN 2045-8827, Vol. 7, no 1, article id e00528Article in journal (Refereed) Published
Abstract [en]

Salmonella enterica subspecies enterica serovar Typhimurium is the most common zoonotic pathogen in Bulgaria. To allow efficient outbreak investigations and surveillance in the food chain, accurate and discriminatory methods for typing are needed. This study evaluated the use of multiple-locus variable-number of tandem repeats analysis (MLVA) and compared results with antimicrobial resistance (AMR) determinations for 100 S. Typhimurium strains isolated in Bulgaria during 2008–2012 (50 veterinary/ food and 50 human isolates). Results showed that isolates were divided into 80 and 34 groups using MLVA and AMR, respectively. Simpson’s index of diversity was determined to 0.994 ± 0.003 and 0.945 ± 0.012. The most frequently encountered MLVA profiles were 3-11-9-NA-211 (n = 5); 3-12-9-NA-211 (n = 3); 3-12-11-21-311 (n = 3); 3-17-10-NA-311 (n = 3); 2-20-9-7-212 (n = 3); and 2-23-NA-NA-111 (n = 3). No clustering of isolates related to susceptibility/resistance to antimicrobials, source of isolation, or year of isolation was observed. Some MLVA types were found in both human and veterinary/food isolates, indicating a possible route of transmission. A majority (83%) of the isolates were found to be resistant against at least one antimicrobial and 44% against ≥4 antimicrobials. Further studies are needed to verify MLVA usefulness over a longer period of time and with more isolates, including outbreak strains.

Keywords
antimicrobial resistance, laboratory surveillance, MLVA, public health, Salmonella genetic
National Category
Microbiology
Identifiers
urn:nbn:se:ri:diva-31195 (URN)10.1002/mbo3.528 (DOI)2-s2.0-85042273394 (Scopus ID)
Available from: 2017-08-24 Created: 2017-08-24 Last updated: 2023-08-02Bibliographically approved
Fachmann, M. S., Löfström, C., Hoorfar, J., Hansen, F., Christensen, J., Mansdal, S. & Josefsen, M. H. (2017). Detection of Salmonella in meat in less than 5 hours by a low-cost and non-complex sample preparation method.. Applied and Environmental Microbiology, 83(5), Article ID e03151-16.
Open this publication in new window or tab >>Detection of Salmonella in meat in less than 5 hours by a low-cost and non-complex sample preparation method.
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2017 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 83, no 5, article id e03151-16Article in journal (Refereed) Published
Abstract [en]

Salmonella is recognised as one of the most important foodborne bacteria, and has a wide health and socioeconomical impact worldwide. Fresh pork meat is one of the main sources of Salmonella and efficient and fast methods for detection are therefore necessary. Current methods for Salmonella detection in fresh meat usually include >16 h of culture enrichment, in few cases < 12 h, thus requiring at least two working shifts. Here, we report a rapid (< 5 h) and high throughput method, for screening of Salmonella in samples from fresh pork meat, consisting of a 3-h enrichment in standard buffered peptone water, and a real-time PCR compatible sample preparation method, based on filtration, centrifugation, and enzymatic digestion, followed by fast cycling real-time PCR detection. The method was validated in an un-paired, comparative study against the Nordic Committee on Food Analysis (NMKL) reference culture method 187. Pork meat samples (n=140) were either artificially contaminated with Salmonella at levels: 0, 1-10 and 10-100 CFU/25 g, or naturally contaminated. Cohen's Kappa for degree of agreement between the rapid method and the reference was 0.64 and the relative accuracy, sensitivity and specificity for the rapid method were 81.4, 95.1 and 97.9 %, respectively. The limit of detection (LOD50) was 8.8 CFU/25 g for the rapid method and 7.7 CFU/25 g for the reference method. Implementation of this method will enable faster release of Salmonella low risk meat, providing savings for meat producers, and help contribute to improved food safety.

IMPORTANCE: While the cost of analysis and hands-on time of the presented rapid method were comparable to reference culture methods, the fast product release by this method can provide the meat industry with a competitive advantage. Not only will the abattoirs save costs for work hours and cold storage; consumers as well as retailers will also benefit from fresher meat with a longer shelf life. Furthermore, the presented sample preparation might be adjusted for application in detection of other pathogenic bacteria in different sample types.

National Category
Microbiology
Identifiers
urn:nbn:se:ri:diva-28015 (URN)10.1128/AEM.03151-16 (DOI)27986726 (PubMedID)2-s2.0-85013498114 (Scopus ID)
Available from: 2017-02-10 Created: 2017-02-10 Last updated: 2019-02-01Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-6492-1245

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