Change search
Link to record
Permanent link

Direct link
BETA
Publications (5 of 5) Show all publications
Pedersen, E. & Fant, K. (2018). Guidance Document on Good In Vitro Method Practices (GIVIMP): Series on Testing and Assessment No. 286. OECD Publishing
Open this publication in new window or tab >>Guidance Document on Good In Vitro Method Practices (GIVIMP): Series on Testing and Assessment No. 286
2018 (English)Other, Policy document (Other academic)
Place, publisher, year, pages
OECD Publishing, 2018. p. 264
Series
OECD Series on Testing and Assessment ; ENV/JM/MONO(2018)19
Keywords
GIVIMP, 3R, validation, alternative methods
National Category
Cell Biology Biochemistry and Molecular Biology Microbiology
Identifiers
urn:nbn:se:ri:diva-35092 (URN)
Available from: 2018-08-30 Created: 2018-08-30 Last updated: 2019-06-28Bibliographically approved
De Jong, W. H., Hoffmann, S., Lee, M., Kandárová, H., Pellevoisin, C., Haishima, Y., . . . Coleman, K. P. (2018). Round robin study to evaluate the reconstructed human epidermis (RhE) model as an in vitro skin irritation test for detection of irritant activity in medical device extracts. Toxicology in Vitro, 50, 439-449
Open this publication in new window or tab >>Round robin study to evaluate the reconstructed human epidermis (RhE) model as an in vitro skin irritation test for detection of irritant activity in medical device extracts
Show others...
2018 (English)In: Toxicology in Vitro, ISSN 0887-2333, E-ISSN 1879-3177, Vol. 50, p. 439-449Article in journal (Refereed) Published
Abstract [en]

Assessment of skin irritation is an essential component of the safety evaluation of medical devices. OECD Test Guideline 439 describes the use of reconstructed human epidermis (RhE) as an in vitro test system for classification of skin irritation by neat chemicals. An international round robin study was conducted to evaluate the RhE method for determination of skin irritant potential of medical device extracts. Four irritant polymers and three non-irritant controls were obtained or developed that had demonstrated their suitability to act as positive or negative test samples. The RhE tissues (EpiDerm™ and SkinEthic™ RHE) were dosed with 100 μL aliquots of either saline or sesame oil extract. Incubation times were 18 h (EpiDerm™) and 24 h (SkinEthic™ RHE). Cell viability reduction > 50% was indicative of skin irritation. Both the EpiDerm™ and SkinEthic™ RHE tissues were able to correctly identify virtually all of the irritant polymer samples either in the saline, sesame oil or both solvent extracts. Our results indicate that RhE tissue models can detect the presence of strong skin irritants at low levels in dilute medical device polymer extracts. Therefore, these models may be suitable replacements for the rabbit skin irritation test to support the biological evaluation of medical devices.

Keywords
Alternative testing, In vitro, Irritation, Medical devices, Reconstructed human epidermis
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-33262 (URN)10.1016/j.tiv.2018.01.001 (DOI)2-s2.0-85040654993 (Scopus ID)
Available from: 2018-02-12 Created: 2018-02-12 Last updated: 2018-12-20Bibliographically approved
Spezzati, G., Fant, K., Ahniyaz, A., Lundin Johnson, M., Hensen, E. J. M., Langermans, H. & Hofmann, J. P. (2017). Synthesis, Physicochemical Characterization, and Cytotoxicity Assessment of CeO2 Nanoparticles with Different Morphologies. European Journal of Inorganic Chemistry, 2017(25), 3184-3190
Open this publication in new window or tab >>Synthesis, Physicochemical Characterization, and Cytotoxicity Assessment of CeO2 Nanoparticles with Different Morphologies
Show others...
2017 (English)In: European Journal of Inorganic Chemistry, ISSN 1434-1948, E-ISSN 1099-1948, Vol. 2017, no 25, p. 3184-3190Article in journal (Refereed) Published
Abstract [en]

With the growing use of nanomaterials, it is essential to carefully determine whether they represent a risk for potential users. So far, validated stand-alone methods that allow a proper risk assessment are still rare. In the present study, the cytotoxicity of CeO2 nanoparticles has been assessed. For this purpose, a variety of well-defined CeO2 nanoparticles has been prepared by using either hydrothermal synthesis or flame spray pyrolysis (FSP), resulting in nanoparticles of different morphologies and sizes. The FSP technique is known to produce particles of a very small size (in the range of nanometers), which can easily become airborne. We employed a characterization procedure that makes use of physicochemical techniques, comprising N2 physisorption, XRD, TEM, as well as ζ-potential and surface-charge measurements. The cytotoxicity of the nanoparticles was evaluated in vitro on two different human lung cell lines (A549 and MRC-5). The tests showed that, despite the differences in surface properties, size, and morphologies, neither of the CeO2 samples gave rise to a cytotoxic response.

Place, publisher, year, edition, pages
Wiley-VCH Verlag, 2017
Keywords
Cerium, Cytotoxicity, Flame spray pyrolysis, Nanoparticles, Nanotoxicity
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-30267 (URN)10.1002/ejic.201700248 (DOI)2-s2.0-85024122445 (Scopus ID)
Available from: 2017-08-11 Created: 2017-08-11 Last updated: 2019-01-16Bibliographically approved
Fant, K. (2014). Enhanced cellular uptake of antisecretory peptide AF-16 through proteoglycan binding (ed.). Biochemistry, 53(41), 6566-6573
Open this publication in new window or tab >>Enhanced cellular uptake of antisecretory peptide AF-16 through proteoglycan binding
2014 (English)In: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 53, no 41, p. 6566-6573Article in journal (Refereed) Published
Abstract [en]

Peptide AF-16, which includes the active site of Antisecretory Factor protein, has antisecretory and anti-inflammatory properties, making it a potent drug candidate for treatment of secretory and inflammatory diseases such as diarrhea, inflammatory bowel diseases, and intracranial hypertension. Despite remarkable physiological effects and great pharmaceutical need for drug discovery, very little is yet understood about AF-16 mechanism of action. In order to address interaction mechanisms, we investigated the binding of AF-16 to sulfated glycosaminoglycan, heparin, with focus on the effect of pH and ionic strength, and studied the influence of cell-surface proteoglycans on cellular uptake efficiency. Confocal laser scanning microscopy and flow cytometry experiments on wild type and proteoglycan-deficient Chinese hamster ovary cells reveal an endocytotic nature of AF-16 cellular uptake that is, however, less efficient for the cells lacking cell-surface proteoglycans. Isothermal titration calorimetry provides quantitative thermodynamic data and evidence for that the peptide affinity to heparin increases at lower pH and ionic strength. Experimental data, supported by theoretical modeling, of peptide-glycosaminoglycan interaction indicate that it has a large electrostatic contribution, which will be enhanced in diseases accompanied by decreased pH and ionic strength. These observations show that cell-surface proteoglycans are of general and crucial importance for the antisecretory and anti-inflammatory activities of AF-16. 

National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-6707 (URN)10.1021/bi5010377 (DOI)2-s2.0-84908032718 (Scopus ID)23705 (Local ID)23705 (Archive number)23705 (OAI)
Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2019-08-08Bibliographically approved
Fant, K. (2012). Functionalization with C-terminal cysteine enhances transfection efficiency of cell-penetrating peptides through dimer formation (ed.). Biochemical and Biophysical Research Communications - BBRC, 418(3), 469-474
Open this publication in new window or tab >>Functionalization with C-terminal cysteine enhances transfection efficiency of cell-penetrating peptides through dimer formation
2012 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 418, no 3, p. 469-474Article in journal (Refereed) Published
National Category
Natural Sciences
Identifiers
urn:nbn:se:ri:diva-6399 (URN)23909 (Local ID)23909 (Archive number)23909 (OAI)
Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2018-08-20Bibliographically approved
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-4109-319x

Search in DiVA

Show all publications
v. 2.35.9