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Publikationer (10 of 38) Visa alla publikationer
Håkansson, J., Juhlin, O., Hovannisyan, A., Rosendahl, J., Bogestål, Y. & Olmarker, K. (2024). Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study. Journal of Orthopaedics, 47, 23-27
Öppna denna publikation i ny flik eller fönster >>Changes in ion-channels in the dorsal root ganglion after exposure to autologous nucleus pulposus and TNF. A rat experimental study
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2024 (Engelska)Ingår i: Journal of Orthopaedics, ISSN 0972-978X, E-ISSN 2589-9082, Vol. 47, s. 23-27Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Purpose: It is known that contact of nucleus pulposus with the dorsal root ganglion may induce changes in nerve conduction and pain behavior. It has also been suggested that the behavioristic changes are caused by changes in voltage-gated ion channels, which in turn have been upregulated by TNF. Such upregulations have previously been shown for NaV 1.8 and NaV 1.9. In this investigation, we expanded the number of studied ion channels after the application of nucleus pulposus or TNF. Methods: Following removal of the left L4-5 fact joint, a disc puncture was performed and the dorsal root ganglion was exposed to nucleus pulposus (n = 5) and TNF (n = 5). Operated rats without disc puncture served as sham (n = 5) and 5 non-operated (naïve) rats were included. After 24 h, the DRGs were harvested and analyzed by quantitative PCR on validated pre-spotted primer plates displaying genes for 90 voltage-gated ion channels. Results: It was evident that the changes in operated animals were separate from the naïve rats. It was also apparent that gene expression changes in rats with nucleus pulposus or TNF application showed similar trends and were also separated from sham-operated animals. Conclusion: The application of nucleus pulposus and TNF onto the DRG in rats induces comparable changes in gene expression of several ion channels. Since the changes induced by TNF and NP are similar, one might also suspect that TNF mediates the NP-induced changes. However, such a mechanism needs further investigation. © 2023 The Authors

Ort, förlag, år, upplaga, sidor
Reed Elsevier India Pvt. Ltd., 2024
Nationell ämneskategori
Kirurgi
Identifikatorer
urn:nbn:se:ri:diva-68103 (URN)10.1016/j.jor.2023.11.012 (DOI)2-s2.0-85176950867 (Scopus ID)
Forskningsfinansiär
AFA Försäkring
Anmärkning

The institution of the corresponding author (KO) has received funding from AFA Insurance , Stockholm, Sweden.

Tillgänglig från: 2023-12-07 Skapad: 2023-12-07 Senast uppdaterad: 2024-01-22Bibliografiskt granskad
Hui, I., Pasquier, E., Solberg, A., Agrenius, K., Håkansson, J. & Chinga Carrasco, G. (2023). Biocomposites containing poly(lactic acid) and chitosan for 3D printing: Assessment of mechanical, antibacterial and in vitro biodegradability properties. Journal of The Mechanical Behavior of Biomedical Materials, 147, Article ID 106136.
Öppna denna publikation i ny flik eller fönster >>Biocomposites containing poly(lactic acid) and chitosan for 3D printing: Assessment of mechanical, antibacterial and in vitro biodegradability properties
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2023 (Engelska)Ingår i: Journal of The Mechanical Behavior of Biomedical Materials, ISSN 1751-6161, E-ISSN 1878-0180, Vol. 147, artikel-id 106136Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

New bone repair materials are needed for treatment of trauma- and disease-related skeletal defects as they still represent a major challenge in clinical practice. Additionally, new strategies are required to combat orthopedic device-related infections (ODRI), given the rising incidence of total joint replacement and fracture fixation surgeries in increasingly elderly populations. Recently, the convergence of additive manufacturing (AM) and bone tissue engineering (BTE) has facilitated the development of bone healthcare to achieve personalized three-dimensional (3D) scaffolds. This study focused on the development of a 3D printable bone repair material, based on the biopolymers poly(lactic acid) (PLA) and chitosan. Two different types of PLA and chitosan differing in their molecular weight (MW) were explored. The novel feature of this research was the successful 3D printing using biocomposite filaments composed of PLA and 10 wt% chitosan, with clear chitosan entrapment within the PLA matrix confirmed by Scanning Electron Microscopy (SEM) images. Tensile testing of injection molded samples indicated an increase in stiffness, compared to pure PLA scaffolds, suggesting potential for improved load-bearing characteristics in bone scaffolds. However, the potential benefit of chitosan on the biocomposite stiffness could not be reproduced in compression testing of 3D printed cylinders. The antibacterial assays confirmed antibacterial activity of chitosan when dissolved in acetic acid. The study also verified the biodegradability of the scaffolds, with a process producing an acidic environment that could potentially be neutralized by chitosan. In conclusion, the study indicated the feasibility of the proposed PLA/chitosan biocomposite for 3D printing, demonstrating adequate mechanical strength, antibacterial properties and biodegradability, which could serve as a new material for bone repair.

Ort, förlag, år, upplaga, sidor
Elsevier Ltd, 2023
Nyckelord
3D printing; Biodegradability; Biopolymers; Compression testing; Injection molding; Lactic acid; Repair; Scaffolds (biology); Scanning electron microscopy; Stiffness; Stiffness matrix; Tensile testing; 3-D printing; 3D-printing; Antibacterials; Biocomposite; Bone repair materials; In-vitro; Mechanical; Poly lactic acid; Poly(lactic acid); Property; Chitosan
Nationell ämneskategori
Polymerteknologi
Identifikatorer
urn:nbn:se:ri:diva-67723 (URN)10.1016/j.jmbbm.2023.106136 (DOI)2-s2.0-85172305781 (Scopus ID)
Forskningsfinansiär
Norges forskningsråd, 337610
Anmärkning

Norges forskningsråd

Tillgänglig från: 2023-11-03 Skapad: 2023-11-03 Senast uppdaterad: 2023-11-21Bibliografiskt granskad
Chinga Carrasco, G., Pasquier, E., Solberg, A., Leirset, I., Stevanic Srndovic, J., Rosendahl, J. & Håkansson, J. (2023). Carboxylated nanocellulose for wound healing applications – Increase of washing efficiency after chemical pre-treatment and stability of homogenized gels over 10 months. Carbohydrate Polymers, 314, Article ID 120923.
Öppna denna publikation i ny flik eller fönster >>Carboxylated nanocellulose for wound healing applications – Increase of washing efficiency after chemical pre-treatment and stability of homogenized gels over 10 months
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2023 (Engelska)Ingår i: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 314, artikel-id 120923Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

To commercialize a biomedical product as a medical device, reproducibility of production and time-stability are important parameters. Studies of reproducibility are lacking in the literature. Additionally, chemical pre-treatments of wood fibres to produce highly fibrillated cellulose nanofibrils (CNF) seem to be demanding in terms of production efficiency, being a bottleneck for industrial upscaling. In this study, we evaluated the effect of pH on the dewatering time and washing steps of 2,2,6,6-Tetramethylpiperidinyloxy (TEMPO)-mediated oxidized wood fibres when applying 3.8 mmol NaClO/g cellulose. The results indicate that the method does not affect the carboxylation of the nanocelluloses, and levels of approximately 1390 μmol/g were obtained with good reproducibility. The washing time of a Low-pH sample was reduced to 1/5 of the time required for washing a Control sample. Additionally, the stability of the CNF samples was assessed over 10 months and changes were quantified, the most pronounced were the increase of potential residual fibre aggregates, reduction of viscosity and increase of carboxylic acid content. The cytotoxicity and skin irritation potential were not affected by the detected differences between the Control and Low-pH samples. Importantly, the antibacterial effect of the carboxylated CNFs against S. aureus and P. aeruginosa was confirmed. © 2023 The Authors

Ort, förlag, år, upplaga, sidor
Elsevier Ltd, 2023
Nyckelord
Antibacterial, Degradation, Hydrolysis, Nanocellulose, TEMPO-oxidized fibres, Wound dressings, Chemical stability, Fibers, Gels, Nanofibers, pH, Production efficiency, Washing, Wood, 2, 2, 6, 6-tetramethylpiperidinyloxy-oxidized fiber, Antibacterials, Cellulose nanofibrils, Chemical pre-treatment, Nano-cellulose, Reproducibilities, Washing efficiency, Woodfiber, Wound healing applications, Carboxylation
Nationell ämneskategori
Pappers-, massa- och fiberteknik
Identifikatorer
urn:nbn:se:ri:diva-64385 (URN)10.1016/j.carbpol.2023.120923 (DOI)2-s2.0-85152907526 (Scopus ID)
Anmärkning

Correspondence Address: Chinga-Carrasco, G.; RISE, Norway; email: gary.chinga.carrasco@rise-pfi.no; Funding details: Norges Forskningsråd, 309178; Funding text 1: The authors thank the Research Council of Norway for funding (OxyPol project - “Oxygenated biopolymers for biomedical applications”, grant no. 309178 ). 

Tillgänglig från: 2023-05-03 Skapad: 2023-05-03 Senast uppdaterad: 2023-11-03Bibliografiskt granskad
Håkansson, J., Jenndahl, L., Simonsson, S., Johansson, M. E., Larsson, K., Strehl, R. & Olsen Ekerhult, T. (2023). De- and recellularized urethral reconstruction with autologous buccal mucosal cells implanted in an ovine animal model. Biomedizinische Technik (Berlin. Zeitschrift)
Öppna denna publikation i ny flik eller fönster >>De- and recellularized urethral reconstruction with autologous buccal mucosal cells implanted in an ovine animal model
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2023 (Engelska)Ingår i: Biomedizinische Technik (Berlin. Zeitschrift), ISSN 1862-278X, E-ISSN 0013-5585Artikel i tidskrift (Refereegranskat) Epub ahead of print
Abstract [en]

Patients with urethral stricture due to any type of trauma, hypospadias or gender dysphoria suffer immensely from impaired capacity to urinate and are in need of a new functional urethra. Tissue engineering with decellularization of a donated organ recellularized with cells from the recipient patient has emerged as a promising alternative of advanced therapy medicinal products. The aim of this pilot study was to develop an ovine model of urethral transplantation and to produce an individualized urethra graft to show proof of function in vivo. Donated urethras from ram abattoir waste were decellularized and further recellularized with autologous buccal mucosa epithelial cells excised from the recipient ram and expanded in vitro. The individualized urethral grafts were implanted by reconstructive surgery in rams replacing 2.5 ± 0.5 cm of the native penile urethra. After surgery optimization, three ram had the tissue engineered urethra implanted for one month and two out of three showed a partially regenerated epithelium. Further adjustments of the model are needed to achieve a satisfactory proof-of-concept; however, we interpret these findings as a proof of principle and a possible path to develop a functional tissue engineered urethral graft with de- and recellularization and regeneration in vivo after transplantation. 

Ort, förlag, år, upplaga, sidor
De Gruyter Open Ltd, 2023
Nyckelord
ATMP, buccal mucosa, decellularization, ovine model, recellularization, regenerative medicine, urethral stricture, Cell engineering, Cells, Cytology, Surgery, Tissue, Tissue regeneration, Animal model, Decellularized, In-vivo
Nationell ämneskategori
Urologi och njurmedicin
Identifikatorer
urn:nbn:se:ri:diva-64335 (URN)10.1515/bmt-2022-0386 (DOI)2-s2.0-85151846769 (Scopus ID)
Anmärkning

 Funding details: VINNOVA, 2017–02130; Funding text 1: Research funding: This study was supported by VINNOVA project CAMP (Contract no. 2017–02130). This study was partly performed by funding from all partners involved with required co-financing.

Tillgänglig från: 2023-04-21 Skapad: 2023-04-21 Senast uppdaterad: 2023-07-06Bibliografiskt granskad
Zhang, Y., Håkansson, J., Fan, Y., Andrén, O. C., San Jacinto García, J., Qin, L., . . . Malkoch, M. (2023). Dendritic Nanogels Directed Dual-Encapsulation Topical Delivery System of Antimicrobial Peptides Targeting Skin Infections. Macromolecular Bioscience
Öppna denna publikation i ny flik eller fönster >>Dendritic Nanogels Directed Dual-Encapsulation Topical Delivery System of Antimicrobial Peptides Targeting Skin Infections
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2023 (Engelska)Ingår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195Artikel i tidskrift (Refereegranskat) Epub ahead of print
Abstract [en]

Antimicrobial peptides (AMPs) are promising antibacterial agents in the fight against multidrug resistant pathogens. However, their application to skin infections is limited by the absence of a realizable topical delivery strategy. Herein, a hybrid hierarchical delivery system for topical delivery of AMPs is accomplished through the incorporation of AMPs into dendritic nanogels (DNGs) and their subsequent embedding into poloxamer gel. The high level of control over the crosslink density and the number of chosen functionalities makes DNGs ideal capsules with tunable loading capacity for DPK-060, a human kininogen-derived AMP. Once embedded into the poloxamer gel, DPK-060 encapsulated in DNGs displays a slower release rate compared to those entrapped directly in the gels. In vitro EpiDerm Skin Irritation Tests show good biocompatibility, while MIC and time-kill curves reveal the potency of the peptide toward Staphylococcus aureus. Anti-infection tests on ex vivo pig skin and in vivo mouse infection models demonstrate that formulations with 0.5% and 1% AMPs significantly inhibit the growth of S. aureus. Similar outcomes are observed for an in vivo mouse surgical site infection model. Importantly, when normalizing the bacteria inhibition to released/free DPK-060 at the wound site, all formulations display superior efficacy compared to DPK-060 in solution. © 2023 The Authors. 

Ort, förlag, år, upplaga, sidor
John Wiley and Sons Inc, 2023
Nyckelord
antimicrobial peptide delivery, dendritic nanogel, DPK-060, poloxamer gel, Antimicrobial agents, Bacteria, Biocompatibility, Mammals, Nanostructured materials, Peptides, Antimicrobial peptide, Dendritics, Nanogels, Peptide delivery, Poloxamer, Topical delivery
Nationell ämneskategori
Naturvetenskap
Identifikatorer
urn:nbn:se:ri:diva-63994 (URN)10.1002/mabi.202200433 (DOI)2-s2.0-85146683018 (Scopus ID)
Anmärkning

Funding details: Knut och Alice Wallenbergs Stiftelse, 2012‐0196, 2017‐0300, 2019‐0002; Funding details: Vetenskapsrådet, VR, 2010–453; Funding details: Seventh Framework Programme, FP7, 60418; Funding details: Barncancerfonden, TJ2017‐0009; Funding text 1: The authors acknowledge the Knut och Alice Wallenberg Foundation (KAW) (Grant numbers: 2012‐0196, 2017‐0300, and 2019‐0002), the Seventh Framework Programme (Grant number: 60418), the Barncancerfonden (Grant number: TJ2017‐0009), and the Swedish Research Council (VR) (Grant number 2010–453) for financial support.

Tillgänglig från: 2023-02-15 Skapad: 2023-02-15 Senast uppdaterad: 2023-05-22Bibliografiskt granskad
Rosendahl, J., Zarna, C., Håkansson, J. & Chinga-Carrasco, G. (2023). Gene-Expression Analysis of Human Fibroblasts Affected by 3D-Printed Carboxylated Nanocellulose Constructs. Bioengineering, 10(1), Article ID 121.
Öppna denna publikation i ny flik eller fönster >>Gene-Expression Analysis of Human Fibroblasts Affected by 3D-Printed Carboxylated Nanocellulose Constructs
2023 (Engelska)Ingår i: Bioengineering, E-ISSN 2306-5354, Vol. 10, nr 1, artikel-id 121Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Three-dimensional (3D) printing has emerged as a highly valuable tool to manufacture porous constructs. This has major advantages in, for example, tissue engineering, in which 3D scaffolds provide a microenvironment with adequate porosity for cell growth and migration as a simulation of tissue regeneration. In this study, we assessed the suitability of three cellulose nanofibrils (CNF) that were obtained through 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO)-mediated oxidation. The CNFs were obtained by applying three levels of carboxylation, i.e., 2.5, 3.8, and 6.0 mmol sodium hypochlorite (NaClO) per gram of cellulose. The CNFs exhibited different nanofibrillation levels, affecting the corresponding viscosity and 3D printability of the CNF gels (0.6 wt%). The scaffolds were manufactured by micro-extrusion and the nanomechanical properties were assessed with nanoindentation. Importantly, fibroblasts were grown on the scaffolds and the expression levels of the marker genes, which are relevant for wound healing and proliferation, were assessed in order to reveal the effect of the 3D-scaffold microenvironment of the cells. © 2023 by the authors.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
3D-printing, characterization, gene expression, nanocellulose, wound dressings
Nationell ämneskategori
Biomaterialvetenskap
Identifikatorer
urn:nbn:se:ri:diva-63992 (URN)10.3390/bioengineering10010121 (DOI)2-s2.0-85146750909 (Scopus ID)
Anmärkning

Funding details: 283895, 309178; Funding details: European Commission, EC; Funding details: Norges Forskningsråd, MNET17/NMCS-1204; Funding text 1: The authors acknowledge the European Commission and the Research Council of Norway for funding part of this work through the MANUNET III program (project no. MNET17/NMCS-1204), the MedIn project (grant no. 283895), “New functionalized medical devices for surgical interventions in the pelvic cavity” and the OxyPol project (“Oxygenated biopolymers for biomedical applications”, grant no. 309178).

Tillgänglig från: 2023-02-15 Skapad: 2023-02-15 Senast uppdaterad: 2023-05-22Bibliografiskt granskad
Österberg, K., Bogestål, Y., Jenndahl, L., Gustafsson-Hedberg, T., Synnergren, J., Holmgren, G., . . . Håkansson, J. (2023). Personalized tissue-engineered veins - long term safety, functionality and cellular transcriptome analysis in large animals. Biomaterials Science, 11(11), 3860-3877
Öppna denna publikation i ny flik eller fönster >>Personalized tissue-engineered veins - long term safety, functionality and cellular transcriptome analysis in large animals
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2023 (Engelska)Ingår i: Biomaterials Science, ISSN 2047-4830, E-ISSN 2047-4849, Vol. 11, nr 11, s. 3860-3877Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Tissue engineering is a promising methodology to produce advanced therapy medicinal products (ATMPs). We have developed personalized tissue engineered veins (P-TEV) as an alternative to autologous or synthetic vascular grafts utilized in reconstructive vein surgery. Our hypothesis is that individualization through reconditioning of a decellularized allogenic graft with autologous blood will prime the tissue for efficient recellularization, protect the graft from thrombosis, and decrease the risk of rejection. In this study, P-TEVs were transplanted to vena cava in pig, and the analysis of three veins after six months, six veins after 12 months and one vein after 14 months showed that all P-TEVs were fully patent, and the tissue was well recellularized and revascularized. To confirm that the ATMP product had the expected characteristics one year after transplantation, gene expression profiling of cells from P-TEV and native vena cava were analyzed and compared by qPCR and sequencing. The qPCR and bioinformatics analysis confirmed that the cells from the P-TEV were highly similar to the native cells, and we therefore conclude that P-TEV is functional and safe in large animals and have high potential for use as a clinical transplant graft.

Ort, förlag, år, upplaga, sidor
NLM (Medline), 2023
Nyckelord
animal, endothelium cell, gene expression profiling, pig, procedures, tissue engineering, transplantation, vein, Animals, Endothelial Cells, Swine, Veins
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
urn:nbn:se:ri:diva-65415 (URN)10.1039/d2bm02011d (DOI)2-s2.0-85160870522 (Scopus ID)
Anmärkning

This study was supported by Vinnova project CAMP (contract no. 2017-02130), a common call by VINNOVA and Vetenskapsrådet: Biologcal pharmaseuticals (Dnr 2017-02983),by University of Skövde under grants from the Swedish Knowledge Foundation [#2016-0330, #2020-0014] and Västra Götalandsregionen (consultant check).

Tillgänglig från: 2023-06-15 Skapad: 2023-06-15 Senast uppdaterad: 2023-12-22Bibliografiskt granskad
Pasquier, E., Rosendahl, J., Solberg, A., Ståhlberg, A., Håkansson, J. & Chinga Carrasco, G. (2023). Polysaccharides and Structural Proteins as Components in Three-Dimensional Scaffolds for Breast Cancer Tissue Models: A Review. Bioengineering, 10(6), Article ID 682.
Öppna denna publikation i ny flik eller fönster >>Polysaccharides and Structural Proteins as Components in Three-Dimensional Scaffolds for Breast Cancer Tissue Models: A Review
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2023 (Engelska)Ingår i: Bioengineering, E-ISSN 2306-5354, Vol. 10, nr 6, artikel-id 682Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Breast cancer is the most common cancer among women, and even though treatments are available, efficiency varies with the patients. In vitro 2D models are commonly used to develop new treatments. However, 2D models overestimate drug efficiency, which increases the failure rate in later phase III clinical trials. New model systems that allow extensive and efficient drug screening are thus required. Three-dimensional printed hydrogels containing active components for cancer cell growth are interesting candidates for the preparation of next generation cancer cell models. Macromolecules, obtained from marine- and land-based resources, can form biopolymers (polysaccharides such as alginate, chitosan, hyaluronic acid, and cellulose) and bioactive components (structural proteins such as collagen, gelatin, and silk fibroin) in hydrogels with adequate physical properties in terms of porosity, rheology, and mechanical strength. Hence, in this study attention is given to biofabrication methods and to the modification with biological macromolecules to become bioactive and, thus, optimize 3D printed structures that better mimic the cancer cell microenvironment. Ink formulations combining polysaccharides for tuning the mechanical properties and bioactive polymers for controlling cell adhesion is key to optimizing the growth of the cancer cells. © 2023 by the authors.

Ort, förlag, år, upplaga, sidor
MDPI, 2023
Nyckelord
3D bioprinting, biopolymers, breast cancer models, cells microenvironment
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
urn:nbn:se:ri:diva-65684 (URN)10.3390/bioengineering10060682 (DOI)2-s2.0-85163723963 (Scopus ID)
Anmärkning

Correspondence Address: G. Chinga-Carrasco; RISE PFI AS, Trondheim, Høgskoleringen 6b, NO-7491, Norway; E.P.: A.S. (Amalie Solberg), and G.C.-C. thank the Research Council of Norway and bioMAT4EYE project (Grant 337610) for funding part of this work. A.S. (Anders Ståhlberg) is funded by Region Västra Götaland, Swedish Cancer Society (2022-2080), Swedish Childhood Cancer Foundation (2022-0030), Swedish Research Council (2021-01008); the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965065), Sweden’s Innovation Agency and the Sjöberg Foundation. J.H. and J.R. are funded by the Swedish Foundation for Strategic Research (FID15-0008), Sweden’s Innovation Agency (2017-03737 and 2021-04484) and Region Västra Götalandsregionen (RUN 2018-00017).

Tillgänglig från: 2023-08-07 Skapad: 2023-08-07 Senast uppdaterad: 2023-11-03Bibliografiskt granskad
Kjellin, P., Danielsson, K., Håkansson, J., Agrenius, K., Andersson, T. & Stenlund, P. (2022). Biomechanical and histomorphometric evaluation of skin integration on titanium and PEEK implants with different surface treatments. Journal of materials science. Materials in medicine, 33(10), Article ID 68.
Öppna denna publikation i ny flik eller fönster >>Biomechanical and histomorphometric evaluation of skin integration on titanium and PEEK implants with different surface treatments
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2022 (Engelska)Ingår i: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 33, nr 10, artikel-id 68Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Percutaneous implants are frequently affected by bacterial growth at the skin-implant interface. Integration between implant and surrounding skin is important to prevent bacteria from spreading to the underlying tissue. The standard method to evaluate skin-implant integration is by histomorphometry on samples which have been placed in tissue grown in vivo or ex vivo. In this study, a biomechanical method was developed and evaluated. The integration of implants into porcine skin was studied in an ex vivo model, where pig skin samples were cultivated in a nutrient solution. Cylindrical shaped implants, consisting of polyether ether ketone (PEEK) and titanium (Ti) with different surface treatments, were implanted in the skin tissue and the skin was grown in nutrient solution for 2 weeks. The implants were then extracted from the implantation site and the mechanical force during extraction was measured as a quantitative assessment of skin-implant integration. Implants from each group were also processed for histomorphometry and the degree of epidermal downgrowth (ED) and tissue to implant contact (TIC) was measured. A higher mean pullout force was observed for the PEEK implants compared to the Ti implants. Applying nanosized hydroxyapatite (HA) on Ti and PEEK increased the pullout force compared to uncoated controls, 24% for machined and 70% for blasted Ti, and 51% for machined PEEK. Treatment of Ti and PEEK with nanosized zirconium phosphate (ZrP) did not increase the pullout force. The histomorphometry analysis showed correlation between ED and pullout force, where the pullout force was inversely proportional to ED. For TIC, no significant differences were observed between the groups of same material (i.e. Ti, Ti+HA, Ti+ZrP, and PEEK, PEEK + HA, PEEK + ZrP), but it was significantly higher for PEEK compared to Ti. Scanning electron microscopy analysis was done on samples before and after the pullout tests, showing that the ZrP coating was unaffected by the 2 week ex vivo implantation and pullout procedure, no dissolution or detachment of the coating was observed. For the HA coating, a loss of coating was seen on approximately 5% of the total surface area of the implant. [Figure not available: see fulltext.] © 2022, The Author(s).

Ort, förlag, år, upplaga, sidor
Springer, 2022
Nyckelord
Bacteria, Biocompatibility, Biomechanics, Coatings, Ethers, Integration, Ketones, Mammals, Nutrients, Scanning electron microscopy, Tissue, Titanium carbide, Bacterial growth, Ex-vivo, Histomorphometry, Implant interfaces, In-vivo, Nutrient solution, Pullout force, Skin implants, Titania, Zirconium phosphate, Hydroxyapatite, nanohydroxyapatite, nanomaterial, polyetheretherketone, titanium, unclassified drug, benzophenone derivative, ether derivative, ketone, macrogol, polymer, animal experiment, animal tissue, Article, bone tissue, coating (procedure), controlled study, dissolution, ex vivo study, force, implantation, morphometry, nonhuman, quantitative analysis, surface area, surface property, Yorkshire pig, animal, osseointegration, pig, tooth implant, Animals, Benzophenones, Dental Implants, Durapatite, Polyethylene Glycols, Polymers, Surface Properties, Swine
Nationell ämneskategori
Annan medicinsk bioteknologi
Identifikatorer
urn:nbn:se:ri:diva-61212 (URN)10.1007/s10856-022-06687-y (DOI)2-s2.0-85139239686 (Scopus ID)
Anmärkning

Funding details: 214487; Funding details: Sahlgrenska Akademin; Funding text 1: Toponova AB, Halmstad, Sweden, is acknowledged for the interferometry measurements. Petra Hammarström Johansson at the Institute of Odontology, Sahlgrenska Academy, Gothenburg, is acknowledged for the histology processing. The study was partly financed by Region Västra Götaland FoU-card advanced (Dnr 214487); Funding text 2: Toponova AB, Halmstad, Sweden, is acknowledged for the interferometry measurements. Petra Hammarström Johansson at the Institute of Odontology, Sahlgrenska Academy, Gothenburg, is acknowledged for the histology processing. The study was partly financed by Region Västra Götaland FoU-card advanced (Dnr 214487)

Tillgänglig från: 2022-12-02 Skapad: 2022-12-02 Senast uppdaterad: 2024-01-02Bibliografiskt granskad
Garre, E., Gustafsson, A., Leiva, M., Håkansson, J., Ståhlberg, A., Kovács, A. & Landberg, G. (2022). Breast Cancer Patient-Derived Scaffolds Can Expose Unique Individual Cancer Progressing Properties of the Cancer Microenvironment Associated with Clinical Characteristics. Cancers, 14(9), Article ID 2172.
Öppna denna publikation i ny flik eller fönster >>Breast Cancer Patient-Derived Scaffolds Can Expose Unique Individual Cancer Progressing Properties of the Cancer Microenvironment Associated with Clinical Characteristics
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2022 (Engelska)Ingår i: Cancers, ISSN 2072-6694, Vol. 14, nr 9, artikel-id 2172Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Breast cancer is a heterogeneous disease in terms of cellular and structural composition, and besides acquired aggressive properties in the cancer cell population, the surrounding tumor microenvironment can affect disease progression and clinical behaviours. To specifically decode the clinical relevance of the cancer promoting effects of individual tumor microenvironments, we performed a comprehensive test of 110 breast cancer samples using a recently established in vivo-like 3D cell culture platform based on patient-derived scaffolds (PDSs). Cell-free PDSs were recellularized with three breast cancer cell lines and adaptation to the different patient-based microenvironments was monitored by quantitative PCR. Substantial variability in gene expression between individual PDS cultures from different patients was observed, as well as between different cell lines. Interestingly, specific gene expression changes in the PDS cultures were significantly linked to prognostic features and clinical information from the original cancer. This link was even more pronounced when ERα-status of cell lines and PDSs matched. The results support that PDSs cultures, including a cancer cell line of relevant origin, can monitor the activity of the tumor microenvironment and reveal unique information about the malignancy-inducing properties of the individual cancer niche and serve as a future complementary diagnostic tool for breast cancer. © 2022 by the authors.

Ort, förlag, år, upplaga, sidor
MDPI, 2022
Nyckelord
breast cancer, cancer stem cells, patient-derived scaffolds, translational research, tumor microenvironment
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
urn:nbn:se:ri:diva-59228 (URN)10.3390/cancers14092172 (DOI)2-s2.0-85128735643 (Scopus ID)
Anmärkning

Funding details: 2017-03737; Funding details: 965065, 965580; Funding details: Cancerfonden, 19-0306, 19-0317; Funding details: Vetenskapsrådet, VR, 2019-01273, 2020-04141; Funding text 1: This research was funded by V?stra G?taland Regional Council Sweden, Swedish Research Council (2019-01273 and 2020-04141), Sweden?s Innovation Agency Vinnova (2017-03737), Swedish Cancer Society (19-0317 and 19-0306) and the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965580 and 965065).; Funding text 2: Funding: This research was funded by Västra Götaland Regional Council Sweden, Swedish Research Council (2019-01273 and 2020-04141), Sweden’s Innovation Agency Vinnova (2017-03737), Swedish Cancer Society (19-0317 and 19-0306) and the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement (965580 and 965065).

Tillgänglig från: 2022-06-10 Skapad: 2022-06-10 Senast uppdaterad: 2023-05-22Bibliografiskt granskad
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-4270-8475

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